This study was designed to evaluate the sensitivities of diverse cell lines on DNA damage effects and genotoxic effects of three brominated flame retardants (BFRs) and three metal ions (Cu²⁺, Cd²⁺, Hg²⁺) by comet assay. First, THP-1 was identified as the most sensitive cell line in terms of DNA damage among 11 kinds of cells screened. Accordingly, the THP-1 cell line was used as a model in subsequent single/combined genotoxicity tests. Single exposure tests to BFRs or metal ions revealed that the DNA damage effects increased with increasing exposure concentration. In combined exposure tests, BFRs (at concentrations of 1/2 EC₅₀) were deployed in combination with different concentrations of Cu²⁺, Cd²⁺, or Hg²⁺. The results showed that the % tail DNA values were significantly increased by most mixtures. Our findings on combined toxic effects by comet assay provide valuable information for setting valid environmental safety evaluation standards.

本研究旨在通过彗星试验评估各种细胞系对三种溴化阻燃剂（BFR）和三种金属离子（Cu 2 ⁺，Cd 2 ⁺，Hg ²⁺）的DNA损伤效应和遗传毒性效应的敏感性。首先，就所筛选的11种细胞中的DNA损伤而言，THP-1被确定为最敏感的细胞系。因此，THP-1细胞系在随后的单次/联合遗传毒性试验中用作模型。对BFR或金属离子的单次暴露测试表明，DNA损伤效应随暴露浓度的增加而增加。在联合暴露测试中，BFR（浓度为1/2 EC ₅₀）与不同浓度的Cu 2 ⁺，Cd结合使用²⁺或Hg ²⁺。结果表明，大多数混合物均显着提高了尾巴DNA百分比值。我们对彗星分析的综合毒性影响的发现为设定有效的环境安全评估标准提供了有价值的信息。