Abstract Background To identify differentially expressed genes (DEGs) between muscle and adipose in cattle, we analyzed the data from the RNA sequencing of three Angus × Qinchuan crossbred cattle. Results Searched the Gene Expression Omnibus (GEO) for a microarray dataset of Yan yellow cattle, GSE49992. After the DEGs were identified, we used STRING and Cytoscape to construct a protein–protein interaction (PPI) network, subsequently analyzing the major modules of key genes. In total, 340 DEGs were discovered, including 21 hub genes, which were mainly enriched in muscle contraction, skeletal muscle contraction, troponin complex, lipid particle, Z disc, tropomyosin binding, and actin filament binding. Conclusions In summary, these genes can be regarded as candidate biomarkers for the regulation of muscle and adipose development. How to cite: Wang S, Raza SHA, Mei C, et al. Transcriptome profiling reveals differential expression of genes potentially involved in muscle and adipose tissue development of cattle. Electron J Biotechnol 2020;48. https://doi.org/10.1016/j.ejbt.2020.09.004 .

中文翻译：

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转录组分析揭示了可能参与牛肌肉和脂肪组织发育的基因的差异表达

摘要 背景为了鉴定牛肌肉和脂肪之间的差异表达基因（DEGs），我们分析了三头安格斯×秦川杂交牛的RNA测序数据。结果 在基因表达综合 (GEO) 中搜索了燕黄牛的微阵列数据集 GSE49992。确定DEGs后，我们使用STRING和Cytoscape构建蛋白质-蛋白质相互作用（PPI）网络，随后分析关键基因的主要模块。总共发现了340个DEGs，包括21个hub基因，主要富集于肌肉收缩、骨骼肌收缩、肌钙蛋白复合物、脂质颗粒、Z盘、原肌球蛋白结合和肌动蛋白丝结合。结论 总之，这些基因可被视为调节肌肉和脂肪发育的候选生物标志物。如何引用：Wang S, Raza SHA、Mei C 等。转录组分析揭示了可能参与牛肌肉和脂肪组织发育的基因的差异表达。电子 J 生物技术 2020；48。https://doi.org/10.1016/j.ejbt.2020.09.004。