With the aim to widen the current knowledge of toxinological implications of bites from rear-fanged snakes and biological roles of their venoms, this study focuses on the biochemical composition and toxic effects of the venom of Leptodeira annulata pulchriceps from Argentina. We analyzed the protein composition by electrophoresis and mass spectrometry, and enzymatic properties by quantitative assays on different substrates. Additionally, we evaluated local and systemic toxicity in mice, and tested its cross-reactivity with elapid and viperid antivenoms used in Argentina. This venom showed features reminiscent of venoms from snakes of Bothrops genus, containing components ranging from ~17 to 75 kDa, which are mainly tissue-damaging toxins such as proteinases. Although showing low lethality to mice (LD₅₀ = 20 μg/g body weight), prominent hemorrhage developed locally in mice intramuscularly and intradermally injected with the venom, and the minimum hemorrhagic dose was found to be 12.7 μg/mouse. This study is the first comprehensive investigation of the venom of L. a. pulchriceps, and sheds new light on differences between this and those of the other two subspecies of L. annulata. Additionally, the study provides new insights into the venom components of “colubrid” snakes, advocating for considering bites from this rich diversity of snakes as a public health problem that needs to be addressed worldwide.

为了拓宽对后排蛇咬伤的毒理学意义及其毒液的生物学作用的了解，本研究着重于阿根廷黑翅病小菜蛾的毒液的生化成分和毒性作用。我们通过电泳和质谱分析了蛋白质组成，并通过在不同底物上进行定量分析来分析了酶学性质。此外，我们评估了小鼠的局部和全身毒性，并测试了其与阿根廷使用的弹性和抗蛇毒抗毒剂的交叉反应性。这显示出毒液毒液让人联想到的特征从蛇矛头属，包含约17至75 kDa的成分，主要是破坏组织的毒素，如蛋白酶。尽管对小鼠的致死性较低（LD ₅₀  = 20μg/ g体重），但在肌肉内和皮内注射毒液的小鼠中局部出血明显，并且最小出血剂量为12.7μg/小鼠。这项研究是对L毒液的首次综合研究。一个。肺炎的发生，并揭示了该病与L. L.其他两个亚种之间的差异。圆环。此外，该研究为“蛇蝎”蛇的毒液成分提供了新见解，主张将这种丰富多样的蛇咬伤考虑为需要全球范围解决的公共卫生问题。