Improved anti-Cutibacterium acnes activity of tea tree oil-loaded chitosan-poly(ε-caprolactone) core-shell nanocapsules,Colloids and Surfaces B: Biointerfaces

当前位置： X-MOL 学术 › Colloids Surf. B Biointerfaces › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Improved anti-Cutibacterium acnes activity of tea tree oil-loaded chitosan-poly(ε-caprolactone) core-shell nanocapsules
Colloids and Surfaces B: Biointerfaces ( IF 5.8 ) Pub Date : 2020-09-19 , DOI: 10.1016/j.colsurfb.2020.111371
Natália Prado da Silva ₁ , Eloá do Carmo Rapozo Lavinas Pereira ₂ , Lucas Mattos Duarte ₃ , Jhamine Caroline de Oliveira Freitas ₄ , Camila Guimarães de Almeida ₅ , Thiago Pereira da Silva ₆ , Rossana C N Melo ₆ , Ana Carolina Morais Apolônio ₇ , Marcone Augusto Leal de Oliveira ₈ , Humberto de Mello Brandão ₅ , Frederico Pittella ₂ , Rodrigo Luiz Fabri ₉ , Guilherme Diniz Tavares ₂ , Priscila de Faria Pinto ₁₀

Affiliation

Protein Structure and Function Study Laboratory, Department of Biochemistry, Institute of Biological Sciences, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil; Laboratory of Nanostructured Systems Development, Department of Pharmaceutical Science, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
Laboratory of Nanostructured Systems Development, Department of Pharmaceutical Science, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
Group of Analytical Chemistry and Chemometrics, Department of Chemistry, Institute of Exact Sciences, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil; Department of Analytical Chemistry, Institute of Chemistry, Fluminense Federal University, Niterói, Rio de Janeiro, 24020-121, Brazil.
Protein Structure and Function Study Laboratory, Department of Biochemistry, Institute of Biological Sciences, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil; Bioactive Natural Products Laboratory, Department of Biochemistry, Institute of Biological Sciences, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
Laboratory of Nanotechnology, Brazilian Agricultural Research Corporation (EMBRAPA), Juiz de Fora, Brazil.
Laboratory of Cellular Biology, Department of Biology, Institute of Biological Sciences, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
Department of Parasitology, Microbiology and Immunology, Institute of Biological Sciences, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
Group of Analytical Chemistry and Chemometrics, Department of Chemistry, Institute of Exact Sciences, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
Bioactive Natural Products Laboratory, Department of Biochemistry, Institute of Biological Sciences, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
Protein Structure and Function Study Laboratory, Department of Biochemistry, Institute of Biological Sciences, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.

The purpose of this study was to develop tea tree oil (TTO)-loaded chitosan-poly(ε-caprolactone) core-shell nanocapsules (NC-TTO-Ch) aiming the topical acne treatment. TTO was analyzed by gas chromatography-mass spectrometry, and nanocapsules were characterized regarding mean particle size (Z-average), polydispersity index (PdI), zeta potential (ZP), pH, entrapment efficiency (EE), morphology by Atomic Force Microscopy (AFM), and anti-Cutibacterium acnes activity. The main constituents of TTO were terpinen-4-ol (37.11 %), γ-terpinene (16.32 %), α-terpinene (8.19 %), ρ-cimene (6.56 %), and α-terpineol (6.07 %). NC-TTO-Ch presented Z-average of 268.0 ± 3.8 nm and monodisperse size distribution (PdI < 0.3). After coating the nanocapsules with chitosan, we observed an inversion in ZP to a positive value (+31.0 ± 1.8 mV). This finding may indicate the presence of chitosan on the nanocapsules' surface, which was corroborated by the AFM images. In addition, NC-TTO-Ch showed a slightly acidic pH (∼5.0), compatible with topical application. The EE, based on Terpinen-4-ol concentration, was approximately 95 %. This data suggests the nanocapsules' ability to reduce the TTO volatilization. Furthermore, NC-TTO-Ch showed significant anti-C. acnes activity, with a 4× reduction in the minimum inhibitory concentration, compared to TTO and a decrease in C. acnes cell viability, with an increase in the percentage of dead cells (17 %) compared to growth control (6.6 %) and TTO (9.7 %). Therefore, chitosan-poly(ε-caprolactone) core-shell nanocapsules are a promising tool for TTO delivery, aiming at the activity against C. acnes for the topical acne treatment.

中文翻译：

茶树油负载壳聚糖-聚（ε-己内酯）核壳纳米胶囊的抗痤疮杆菌痤疮活性提高

这项研究的目的是开发针对局部痤疮治疗的茶树油（TTO）负载的壳聚糖-聚（ε-己内酯）核壳纳米胶囊（NC-TTO-Ch）。通过气相色谱-质谱法分析TTO，并对纳米胶囊的平均粒径（Z-平均值），多分散指数（PdI），ζ电位（ZP），pH，包封效率（EE），形态进行原子力显微镜表征（ AFM）和抗痤疮杆菌的活性。TTO的主要成分是萜品烯-4-醇（37.11％），γ-萜品烯（16.32％），α-萜品烯（8.19％），ρ-甲烯（6.56％）和α-松油醇（6.07％）。NC-TTO-Ch的Z均值为268.0±3.8 nm，单分散尺寸分布（PdI <0.3）。用壳聚糖包被纳米胶囊后，我们观察到ZP转化为正值（+31.0±1.8 mV）。这一发现可能表明壳聚糖在纳米胶囊的表面上存在，这被AFM图像证实。此外，NC-TTO-Ch的pH呈弱酸性（〜5.0），与局部应用相容。基于萜品四醇的浓度，EE约为95％。该数据表明纳米胶囊具有减少TTO挥发的能力。此外，与TTO相比，NC-TTO-Ch具有显着的抗痤疮丙酸杆菌活性，最小抑制浓度降低了4倍，痤疮丙酸杆菌减少细胞存活率，与生长对照（6.6％）和TTO（9.7％）相比，死细胞百分比（17％）增加。因此，壳聚糖聚（ε-己内酯）核壳纳米胶囊是一种有前途的TTO传递工具，旨在针对痤疮丙酸杆菌的局部痤疮治疗活性。

更新日期：2020-09-25

点击分享查看原文

点击收藏

阅读更多本刊最新论文

全部期刊列表>>