The use of biodiesel-derived glycerol as a carbon source for single cell oil (SCO) production is a biorefinery engineering strategy that aims to valorize the by-product waste and make the microbial lipid production process more cost-effective. This work aimed to improve the capacity of Yarrowia lipolytica to produce large amounts of lipids and replace genetic engineering by a metabolic approach based on the stimulation of the rate-limiting enzymes and reducing the activation energy thereby increasing the rate of lipids synthesis. The effects of biotin and leucine addition on the lipid content of Y. lipolytica have been investigated. The lipid content of Y. lipolytica was strongly influenced by the addition of biotin. In fact, an increase in biotin concentration from 25 μg/L to 200 μg/L practically increased the lipid concentration up to 15 g/L. Besides, to channel metabolic flux into lipid biosynthesis, the addition of citric acid as lipid precursor led to an increase in total catabolism activation and lipid accumulation to reach around 63% (w/w). The biochemical approach can be a useful target for improving the efficiency of lipid-producing yeast strain rather than genetic engineering.

使用生物柴油来源的甘油作为单细胞油（SCO）生产的碳源是一种生物精炼工程策略，其目的是使副产品废物增值并使微生物脂质生产过程更具成本效益。这项工作旨在提高解脂耶氏酵母产生大量脂质的能力，并通过基于限速酶的刺激并降低活化能的代谢方法替代遗传工程，从而提高脂质合成的速率。研究了生物素和亮氨酸添加对解脂耶氏酵母脂质含量的影响。解脂耶氏酵母的脂质含量受到生物素添加的强烈影响。实际上，生物素浓度从25μg/ L增加到200μg/ L实际上将脂质浓度增加到15 g / L。此外，为了将代谢通量引导到脂质生物合成中，添加柠檬酸作为脂质前体导致总分解代谢活化和脂质积累增加，达到约63％（w / w）。生化方法可能是提高产脂质酵母菌株效率而不是基因工程方法的有用目标。