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The adaptation of lipid profile of human fibroblasts to alginate 2D films and 3D printed scaffolds.
Biochimica et Biophysica Acta (BBA) - General Subjects ( IF 3 ) Pub Date : 2020-09-18 , DOI: 10.1016/j.bbagen.2020.129734
Ilaria Zanotti 1 , Silvia Marando 1 , Giulia Remaggi 1 , Carlo Bergonzi 1 , Franco Bernini 1 , Ruggero Bettini 1 , Lisa Elviri 1
Affiliation  

Background

The investigation of the interactions between cells and active materials is pivotal in the emerging 3D printing-biomaterial application fields. Here, lipidomics has been used to explore the early impact of alginate (ALG) hydrogel architecture (2D films or 3D printed scaffolds) and the type of gelling agent (CaCl2 or FeCl3) on the lipid profile of human fibroblasts.

Methods

2D and 3D ALG scaffolds were prepared and characterized in terms of water content, swelling, mechanical resistance and morphology before human fibroblast seeding (8 days). Using a liquid chromatography-triple quadrupole-tandem mass spectrometry approach, selected ceramides (CER), lysophosphatidylcholines (LPC), lysophosphatidic acids (LPA) and free fatty acids (FFA) were analyzed.

Results

The results showed a clear alteration in the CER expression profile depending of both the geometry and the gelling agent used to prepare the hydrogels. As for LPCs, the main parameter affecting their distribution is the scaffold architecture with a significant decrease in the relative expression levels of the species with higher chain length (C20 to C22) for 3D scaffolds compared to 2D films. In the case of FFAs and LPAs only slight differences were observed as a function of scaffold geometry or gelling agent.

Conclusions

Variations in the cell membrane lipid profile were observed for 3D cell cultures compared to 2D and these data are consistent with activation processes occurring through the mutual interactions between fibroblasts and ALG support. These unknown physiologically relevant changes add insights into the discussion about the relationship between biomaterial and the variations of cell biological functions.



中文翻译:

人成纤维细胞的脂质分布对藻酸盐2D胶片和3D打印支架的适应性。

背景

在新兴的3D打印生物材料应用领域中,细胞与活性材料之间相互作用的研究至关重要。在这里,脂质组学已被用来研究藻酸盐(ALG)水凝胶结构(2D膜或3D打印的支架)的早期影响以及胶凝剂的类型(CaCl 2或FeCl 3)对人成纤维细胞脂质分布的影响。

方法

制备2D和3D ALG支架,并在人类成纤维细胞接种前(8天)根据水含量,溶胀,机械抗性和形态进行表征。使用液相色谱-三重四极杆串联质谱法,分析了选定的神经酰胺(CER),溶血磷脂酰胆碱(LPC),溶血磷脂酸(LPA)和游离脂肪酸(FFA)。

结果

结果表明,取决于用于制备水凝胶的几何形状和胶凝剂,CER表达谱有明显改变。对于LPC,影响其分布的主要参数是支架结构,与2D薄膜相比,3D支架具有较高链长(C20至C22)的物种的相对表达水平显着降低。在FFA和LPA的情况下,仅观察到轻微的差异作为支架几何形状或胶凝剂的函数。

结论

与2D相比,对于3D细胞培养,观察到了细胞膜脂质分布的变化,这些数据与通过成纤维细胞和ALG支持物之间的相互作用发生的激活过程一致。这些未知的生理相关变化为有关生物材料与细胞生物学功能变异之间关系的讨论增添了见识。

更新日期:2020-09-22
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