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Sphingomonas palmae sp. nov. and Sphingomonas gellani sp. nov., endophytically associated phyllosphere bacteria isolated from economically important crop plants.
Antonie van Leeuwenhoek ( IF 2.6 ) Pub Date : 2020-09-19 , DOI: 10.1007/s10482-020-01468-5
Munusamy Madhaiyan 1 , Venkatakrishnan Sivaraj Saravanan 2 , Joseph S Wirth 3 , Tan Hian Hwee Alex 1 , Soo-Jin Kim 4 , Hang-Yeon Weon 4 , Soon-Wo Kwon 4 , William B Whitman 5 , Lianghui Ji 1
Affiliation  

In this study, two endophytic bacterial strains designated JS21-1T and S6-262T isolated from leaves of Elaeis guineensis and stem tissues of Jatropha curcas respectively, were subjected for polyphasic taxonomic approach. On R2A medium, colonies of strains JS21-1T and S6-262T are orange and yellow, respectively. Phylogenetic analyses using 16S rRNA gene sequencing and whole-genome sequences placed the strains in distinct clades but within the genus Sphingomonas. The DNA G + C content of JS21-1T and S6-262T were 67.31 and 66.95%, respectively. Furthermore, the average nucleotide identity and digital DNA-DNA hybridization values of strains JS21-1T and S6-262T with phylogenetically related Sphingomonas species were lower than 95% and 70% respectively. The chemotaxonomic studies indicated that the major cellular fatty acids of the strain JS21-1T were summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), C16:0, and C14:0 2OH; strain S6-262T possessed summed feature 3 (C16:1 ω7c and/or iso-C15:0 2-OH) and summed feature 8 (C18:1 ω6c and/or C18:1 ω7c). The major quinone was Q10, and the unique polyamine observed was homospermidine. The polar lipid profile comprised of mixture of sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and certain uncharacterised phospholipids and lipids. Based on this polyphasic evidence, strains JS21-1T and S6-262T represent two novel species of the genus Sphingomonas, for which the names Sphingomonas palmae sp. nov. and Sphingomonas gellani sp. nov. are proposed, respectively. The type strain of Sphingomonas palmae sp. nov. is JS21-1T (= DSM 27348T = KACC 17591T) and the type strain of Sphingomonas gellani sp. nov. is S6-262T (= DSM 27346T = KACC 17594T).



中文翻译:

棕榈鞘氨醇单胞菌 十一月 和鞘氨醇单胞菌 十一月,从经济上重要的农作物中分离出的与内生植物相关的叶环细菌。

在这项研究中,指定JS21-1 2个内生细菌菌株Ť和S6-262 Ť从叶中分离油棕和茎的组织中麻疯树分别进行用于多相分类的方法。在R2A培养基上,菌株JS21-1 T和S6-262 T的菌落分别为橙色和黄色。使用16S rRNA基因测序和全基因组序列进行系统进化分析,将菌株置于鞘氨醇单胞菌属的不同进化枝中。JS21-1 T和S6-262 T的DNA G + C含量分别为67.31和66.95%。此外,具有系统发育相关鞘氨醇单胞菌属的菌株JS21-1 T和S6-262 T的平均核苷酸同一性和数字DNA-DNA杂交值分别低于95%和70%。所述化学分类研究表明,该菌株JS21-1的主要细胞脂肪酸Ť的总和,特征8(C 18:1个ω 7 ç和/或C 18:1个ω 6C),C 16:0,和C 14:0 2OH; 应变S6-262 Ť具有求和功能3(C 16:1点ω 7c和/或异-C 15:0 2-OH)和求和特征8(C 18:1和 ω 6c和/或C 18:1个 ω 7C)。主醌为Q10,观察到的独特多胺为高嘧啶。极性脂质谱包括鞘磷脂,磷脂酰乙醇胺,磷脂酰甘油,二磷脂酰甘油和某些未表征的磷脂和脂质的混合物。基于此多相证据,菌株JS21-1 T和S6-262 T代表了鞘氨醇单胞菌属的两个新物种,其名称为棕榈鞘氨醇单胞菌。十一月 和鞘氨醇单胞菌 十一月 分别提出。棕榈鞘氨醇单胞菌的类型株sp。十一月 是JS21-1 T(= DSM 27348 T  = KACC 17591 T)和Sphingomonas gellani sp。十一月 是S6-262 T(= DSM 27346 T  = KACC 17594 T)。

更新日期:2020-09-20
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