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Biodegradation and detoxification of the triphenylmethane dye coomassie brilliant blue by the extracellular enzymes from mycelia of Lactarius deliciosus
Frontiers of Chemical Science and Engineering ( IF 4.5 ) Pub Date : 2020-09-18 , DOI: 10.1007/s11705-020-1952-7
Jin Zhao , Qing-Xi Wu , Xiao-Du Cheng , Ting Su , Xiao-Hui Wang , Wen-Na Zhang , Yong-Ming Lu , Yan Chen

Fungi play an important role in dying wastewater treatment. In this work, the mycelia of Lactarius deliciosus exhibited an excellent capacity in decolorizing coomassie brilliant blue (CBB). The results demonstrated that the mycelia could treat CBB with high concentrations over a broad range of pH and temperature. The decolorization rate of 99.19% and the removal rate of 16.31 mg·L−1·h were realized. The mycelia could be recycled from decolorizing process for 19 times, indicating a good re-usability. It verified that the lignin peroxidase (121.65 U·L−1) and manganese peroxidase (36.77 U·L−1) were involved in the degradation and decolorization process of CBB. Toxicity assessments indicated the seed germination rate was up to 82.22% while inhibition to Escherichia coli decreased dramatically and no significant effect on Caenorhabditis elegans growth was found. The removal of CBB was a synergistic process accomplished by adsorption and biodegradation. The mycelia could be used for eco-friendly CBB treatment.



中文翻译:

美味乳杆菌菌丝体胞外酶对三苯基甲烷染料考马斯亮蓝的生物降解和解毒作用

真菌在印染废水处理中起着重要作用。在这项工作中,美味乳杆菌的菌丝体具有出色的使考马斯亮蓝(CBB)脱色的能力。结果表明,菌丝体可以在很宽的pH和温度范围内以高浓度处理CBB。实现了99.19%的脱色率和16.31 mg·L -1 ·h的去除率。该菌丝体可从脱色过程中回收19次,表明具有良好的可重复使用性。验证了木质素过氧化物酶(121.65 U·L -1)和锰过氧化物酶(36.77 U·L -1)参与了CBB的降解和脱色过程。毒性评估表明,种子发芽率高达82.22%,而对大肠杆菌的抑制作用则大大降低,未发现对秀丽隐杆线虫生长的显着影响。CBB的去除是通过吸附和生物降解完成的协同过程。该菌丝体可用于环保的CBB治疗。

更新日期:2020-09-20
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