A novel approach has been developed for the detection of 56 kDa tissue-specific antigen (TSA) gene of Orientia tsutsugamushi a causative agent of scrub typhus disease. The approach was developed by immobilization of 5′ NH2 labeled ssDNA probe selective to 56 kDa TSA gene, to the surface of AuNPs/CNF modified screen-printed electrode. An electrochemical response was recorded with single stranded genomic DNA (ssDNA) of O. tsutsugamushi isolated from patient sample, using cyclic voltammetry and electrochemical impedance spectroscopy. The electrode surface was characterized by Field-Emission Scanning electron microscope (FE-SEM), Fourier Transform Infrared Spectroscopy (FTIR) and Raman Spectroscopy at each step of fabrication. The DNA biosensor shows optimum response within 50–60 s at room temperature (25 ± 3 °C). The sensor shows higher sensitivity [7849 (µA/cm²)/ng DNA], fast response time (60 s), wider linear range (0.04–2.6 ng) with limit of detection of 0.02 ng/µl of ssDNA sample.

的新方法已被开发用于检测 56  kDa的组织-特异性抗原 的（TSA）基因 恙虫病东方体 恙虫病疾病的病原体。该方法是通过将 5' NH2 标记的对 56  kDa  TSA 基因具有选择性的 ssDNA 探针固定到 AuNPs/CNF 修饰的丝网印刷电极表面而开发的。用O. tsutsugamushi 的单链基因组 DNA (ssDNA) 记录电化学响应使用循环伏安法和电化学阻抗谱从患者样本中分离。在制造的每个步骤中，通过场发射扫描电子显微镜（FE-SEM）、傅立叶变换红外光谱（FTIR）和拉曼光谱对电极表面进行表征。DNA 生物传感器在室温 (25 ± 3 °C) 下在 50–60 秒内显示出最佳响应。该传感器显示出更高的灵敏度 [7849 (µA/cm ² )/ng DNA]、快速响应时间 (60 s)、更宽的线性范围 (0.04–2.6 ng)，检测限为 0.02 ng/µl ssDNA 样本。