Background: Gastric cancer (GC) may arise in any region of the stomach. Poorly diagnosed GC results in almost one million mortalities annually worldwide. Kirenol is a bioactive compound present in the Sieges beckia sps.
Objective: In this current, we investigate the anticancer capacity of kirenol against the MNG-stimulated GC in rats via modulating the antioxidants status and inhibition of NF-κB cascade.
Methodology: GC was provoked in the rats via supplementing 100 mg/kg of MNU through the intragastric route for 16 weeks concomitantly with 30 mg/kg of kirenol treatment. The body weight, tumor volume, and incidence of all animals were tabulated every week. The status of gastrin, ALP, LDH, and γ-GT was studied through the ELISA tests. The lipid peroxidation, enzymatic, and nonenzymatic antioxidants were determined via standard procedures. Expression of thioredoxin, glutaredoxin, NF-κB, TNF-α, IL-6, PGE2 was studied through RT-PCR. The gastric mucosa was analyzed microscopically.
Results: Kirenol treatment appreciably improved the body weight and diminished the tumor volume and incidences in the MNG-challenged rats. The lipid peroxidation was diminished and the enzymatic and non-enzymatic antioxidants were improved by the kirenol treatment. Kirenol suppressed the status of serum markers of GC and gastrin, ALP, LDH, and γ-GT. The mRNA expression of thioredoxin, glutaredoxin, NF-κB, TNF-α, IL-6, PGE2 was downregulated via the kirenol in the MNG-challenged rats. Histopathological analysis result also confirmed the therapeutic role of kirenol.
Conclusion: These findings proved that the kirenol appreciably prevented the MNG-triggered GC in rats and it may become a potential drug for the GC treatment in the future.

中文翻译：

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Kirenol通过调节氧化应激和炎症标记物对大鼠N-甲基-N-亚硝基脲诱导的胃癌的保护作用

背景：胃癌（GC）可能出现在胃的任何区域。诊断不佳的GC每年在全球造成近一百万的死亡。Kirenol是存在于Sieges beckia sps中的生物活性化合物。
目的：目前，我们通过调节抗氧化剂的状态和抑制NF-κB级联反应，研究基列醇对MNG刺激的GC大鼠的抗癌能力。
方法：在大鼠中，通过胃内途径补充100 mg / kg的MNU并伴随30 mg / kg的肾上腺素治疗，在16周内激发了GC。每周列出所有动物的体重，肿瘤体积和发生率。通过ELISA试验研究了胃泌素，ALP，LDH和γ-GT的状态。通过标准程序测定脂质过氧化，酶促和非酶促抗氧化剂。通过RT-PCR研究了硫氧还蛋白，戊二醛，NF-κB，TNF-α，IL-6，PGE2的表达。显微镜下分析胃粘膜。
结果：Kirenol治疗可显着改善体重，并减少MNG攻击大鼠的肿瘤体积和发生率。减少的过氧化脂质和酶和非酶的抗氧化剂通过基列诺尔处理得到改善。Kirenol抑制了GC和胃泌素，ALP，LDH和γ-GT的血清标志物的状态。硫氧还蛋白，谷胱甘肽毒素，NF-κB，TNF-α，IL-6，PGE2的mRNA表达通过鼠肾上腺素被MNG攻击的大鼠下调。组织病理学分析结果也证实了肾上腺素的治疗作用。
结论：这些发现证明，基列醇可明显预防大鼠MNG触发的GC，并且可能成为将来治疗GC的潜在药物。