A widely used method to measure the bending rigidity of bilayer membranes is fluctuation spectroscopy, which analyses the thermally-driven membrane undulations of giant unilamellar vesicles recorded with either phase-contrast or confocal microscopy. Here, we analyze the fluctuations of the same vesicle using both techniques and obtain consistent values for the bending modulus. We discuss the factors that may lead to discrepancies.

中文翻译：

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使用共聚焦和相衬显微镜对巨大单层囊泡的波动光谱进行分析。

测量双层膜弯曲刚度的一种广泛使用的方法是波动光谱法，该方法可分析通过相差显微镜或共聚焦显微镜记录的巨大单层囊泡的热驱动膜起伏。在这里，我们使用两种技术分析同一囊泡的波动，并获得弯曲模量的一致值。我们讨论了可能导致差异的因素。