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Illuminating glycoscience: synthetic strategies for FRET-enabled carbohydrate active enzyme probes
RSC Chemical Biology Pub Date : 2020-09-17 , DOI: 10.1039/d0cb00134a
Meenakshi Singh 1, 2, 3, 4, 5 , Michael Watkinson 1, 2, 3, 4, 5 , Eoin M. Scanlan 6, 7, 8, 9 , Gavin J. Miller 1, 2, 3, 4, 5
Affiliation  

Carbohydrates are an essential class of biomolecule and carbohydrate active enzymes (CAZys) catalyse their synthesis, refinement, and degradation, hence contributing an overall regulatory capacity to their underpinning physiological roles. As such, there is a considerable current requirement to be able to monitor, quantify and inhibit CAZy activity. It is here that Förster resonance energy transfer (FRET) is emerging as a powerful tool in enabling this, through synthetic conjugation of appropriate fluorogenic partners into a particular CAZy substrate. In this review we survey recent accomplishments in synthetic methodology for accessing defined carbohydrate structures, suitably equipped with FRET probe capability, followed by their utilisation in studying particular classes of CAZy. The majority of examples concern endo-acting glycosidases, but emergent probes for exo-glycosidases, glycosyl transferases and catalytic antibodies are also examined.

中文翻译:

启发糖科学:FRET功能的碳水化合物活性酶探针的合成策略

碳水化合物是生物分子和碳水化合物活性酶(CAZys)的必不可少的一类,催化它们的合成,精制和降解,因此为它们的基础生理作用提供了全面的调节能力。因此,当前对监视,定量和抑制CAZy活性的要求很高。福斯特共振能量转移(FRET)正是通过将适当的荧光伴侣合成缀合到特定的CAZy底物中而成为一种强大的工具。在这篇综述中,我们调查了合成方法的最新成就,这些方法可用于获得确定的碳水化合物结构,并适当配备FRET探针功能,然后将其用于研究特定类别的CAZy。大多数例子都涉及-作用糖苷酶,但也检查了外切糖苷酶,糖基转移酶和催化抗体的探针。
更新日期:2020-11-03
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