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Enzymatic Activities of Streptomyces spp. Isolated from Natural Habitat of Nipah Worm in Sungai Kakap District, West Kalimantan
IOP Conference Series: Earth and Environmental Science Pub Date : 2020-09-17 , DOI: 10.1088/1755-1315/550/1/012017
A H Yanti 1 , T R Setyawati 1 , R Kurniatuhadi 2
Affiliation  

Streptomyces involves in the degradation process of organic compounds on the soils, including mangrove forests. In this process, Streptomyces secretes several enzymes to convert the substrates into other substances, which will readily be available for detritus feeders, including nipah worms (Namalycastis rhodochorde). This study investigated the enzyme activities of six Streptomyces spp. that has been isolated from mangrove soil in Sungai Kakap District, West Kalimantan. Enzymatic activities were detected on four different media: CMC-congo red agar, skim milk agar, gelatine nutritive agar, starch casein agar. Catalase activity was detected using 3% of H2O2 solution. The results showed that six Streptomyces spp. had proteolytic, cellulolytic, amylolytic, gelatin liquefaction, and catalase activities. Streptomyces NrASA1 has the highest proteolytic activity index values, namely 0.57. Streptomyces NrASA3 and Streptomyces NrASA4 have the highest amylolytic and cellulolytic activity index values, respectively 0.76 and 1.15. The enzymatic activity profile of indigenous Streptomyces spp. can be utilized for the development of feed formula for nipah worm cultivation.



中文翻译:

链霉菌属的酶活性。从西加里曼丹省 Sungai Kakap 区 Nipah 蠕虫的自然栖息地中分离出来

链霉菌参与土壤中有机化合物的降解过程,包括红树林。在这个过程中,链霉菌会分泌几种酶来将底物转化为其他物质,这些物质很容易被碎屑饲养者利用,包括尼帕蠕虫 ( Namalycastis rhodohorde )。本研究调查了六种链霉菌属的酶活性。它是从西加里曼丹省 Sungai Kakap 区的红树林土壤中分离出来的。在四种不同的培养基上检测酶活性:CMC-刚果红琼脂、脱脂牛奶琼脂、明胶营养琼脂、淀粉酪蛋白琼脂。使用 3% 的 H 2 O 2溶液检测过氧化氢酶活性。结果表明,六链霉菌属 具有蛋白水解、纤维素分解、淀粉分解、明胶液化和过氧化氢酶活性。链霉菌NrASA1 具有最高的蛋白水解活性指数值,即 0.57。链霉菌NrASA3 和链霉菌NrASA4 具有最高的淀粉分解和纤维素分解活性指数值,分别为 0.76 和 1.15。本土链霉菌属的酶活性概况。可用于开发尼帕虫养殖饲料配方。

更新日期:2020-09-17
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