SNAI2/SLUG, a metastasis-promoting transcription factor, is a labile protein that is degraded through the ubiquitin proteasome degradation system. Here, we conducted comprehensive gain- and loss-of-function screens using a human DUB cDNA library of 65 genes and an siRNA library of 98 genes, and identified USP20 as a deubiquitinase (DUB) that regulates SNAI2 ubiquitination and stability. Further investigation of USP20 demonstrated its function in promoting migration, invasion, and metastasis of breast cancer. USP20 positively correlates with SNAI2 protein level in breast tumor samples, and higher USP20 expression is associated with poor prognosis in ER⁻ breast cancer patients.

中文翻译：

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去泛素化酶 USP20 通过稳定 SNAI2 促进乳腺癌转移。

SNAI2/SLUG 是一种促进转移的转录因子，是一种不稳定的蛋白质，可通过泛素蛋白酶体降解系统降解。在这里，我们使用包含 65 个基因的人类 DUB cDNA 文库和包含 98 个基因的 siRNA 文库进行了全面的功能获得和丧失筛选，并将 USP20 鉴定为调节 SNAI2 泛素化和稳定性的去泛素化酶 (DUB)。USP20的进一步研究表明其在促进乳腺癌迁移、侵袭和转移方面的作用。USP20 与乳腺肿瘤样本中的 SNAI2 蛋白水平呈正相关，较高的 USP20 表达与 ER ^-乳腺癌患者的不良预后相关。