Objective:

To examine the biochemical and histopathological effects of ischemia/reperfusion (I/R) injury in a ruptured abdominal aortic aneurysm (RAAA) model in rats, and to investigate the potential protective role of resveratrol.

Methods:

Thirty-two male Sprague-Dawley rats were randomly assigned into four groups—control, I/R, sham (I/R + solvent/dimethyl sulfoxide), and I/R + resveratrol. The control group underwent midline laparotomy only. In the other groups, infrarenal vascular clamps were attached following 60-min shock to the abdominal aorta. Ischemia was applied for 60 min followed by reperfusion for 120 min. In the I/R + resveratrol group, intraperitoneal 10 mg/kg resveratrol was administered 15 min prior to ischemia and immediately before reperfusion. The I/R + dimethyl sulfoxide group received dimethyl sulfoxide, and the I/R group was given saline solution. All animals were sacrificed by exsanguination from the carotid artery at the end of the experiment. In addition to histopathological examination of the rat kidney tissues, malondialdehyde, glutathione, catalase, and nitric oxide levels were also investigated.

Results:

A decrease in glutathione, catalase and nitric oxide levels, together with increases in malondialdehyde levels, numbers of apoptotic renal tubular cells, caspase-3 levels, and tubular necrosis scores, were observed in the IR and I/R + dimethyl sulfoxide groups. In contrast, resveratrol increased glutathione, catalase and nitric oxide levels in renal tissues exposed to I/R, while reducing malondialdehyde levels, apoptotic renal tubular cell numbers, caspase-3 levels, and tubular necrosis scores.

Conclusion:

Our findings suggest that resveratrol can be effective against I/R-related acute kidney damage developing during RAAA surgery by reducing oxidative stress and apoptosis.

中文翻译：

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白藜芦醇可预防腹主动脉瘤破裂模型中的急性肾损伤。

客观的：

研究大鼠腹主动脉瘤破裂 (RAAA) 模型缺血/再灌注 (I/R) 损伤的生化和组织病理学影响，并探讨白藜芦醇的潜在保护作用。

方法：

32 只雄性 Sprague-Dawley 大鼠被随机分为四组——对照组、I/R、假手术组（I/R + 溶剂/二甲基亚砜）和 I/R + 白藜芦醇。对照组仅接受中线剖腹手术。在其他组中，在 60 分钟电击后将肾下血管夹连接到腹主动脉。缺血 60 分钟，然后再灌注 120 分钟。在 I/R + 白藜芦醇组中，在缺血前 15 分钟和再灌注前立即腹腔注射 10 mg/kg 白藜芦醇。I/R+二甲亚砜基团接受二甲亚砜，I/R基团接受盐水溶液。在实验结束时从颈动脉放血处死所有动物。除了对大鼠肾脏组织进行组织病理学检查外，丙二醛、谷胱甘肽、过氧化氢酶、

结果：

在 IR 和 I/R + 二甲基亚砜组中观察到谷胱甘肽、过氧化氢酶和一氧化氮水平降低，同时丙二醛水平、凋亡肾小管细胞数量、caspase-3 水平和肾小管坏死评分增加。相比之下，白藜芦醇增加了暴露于 I/R 的肾组织中的谷胱甘肽、过氧化氢酶和一氧化氮水平，同时降低了丙二醛水平、凋亡肾小管细胞数量、caspase-3 水平和肾小管坏死评分。

结论：

我们的研究结果表明，白藜芦醇可以通过减少氧化应激和细胞凋亡来有效对抗 RAAA 手术期间发生的 I/R 相关的急性肾损伤。