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Reversine and herbal Xiang–Sha–Liu–Jun–Zi decoction ameliorate thioacetamide-induced hepatic injury by regulating the RelA/NF-κB/caspase signaling pathway
Open Life Sciences ( IF 2.2 ) Pub Date : 2020-09-15 , DOI: 10.1515/biol-2020-0059
Zhen-Hao Mai 1, 2 , Yu Huang 1, 3 , Di Huang 1, 3 , Zi-Sheng Huang 1, 2 , Zhi-Xiang He 1 , Pei-Lin Li 1 , Shuai Zhang 1, 3 , Jie-Feng Weng 1, 3 , Wei-Li Gu 1, 3
Affiliation  

Abstract This study investigated the anti-fibrotic effects of reversine and Chinese medicine Xiang–Sha–Liu–Jun–Zi decoction (XSLJZD) on thioacetamide (TAA)-induced hepatic injury. Sprague-Dawley rats were intraperitoneally administered with TAA, then injected with reversine intraperitoneally, and/or orally provided with XSLJZD. TAA resulted in liver injury with increases in the liver index and levels of serum aspartate aminotransferase (AST) and alanine aminotransferase. Reversine alleviated the liver index and AST level and improved TAA-induced pathological changes but decreased TAA-induced collagen deposition, and α-smooth muscle actin and transforming growth factor-β1 expression. Reversine also modulated the mRNA levels of inflammatory cytokines, such as RelA, interleukin (IL)-17A, IL-22, IL-1β, IL-6, NLR family pyrin domain containing 3, platelet-derived growth factor, and monocyte chemoattractant protein, and suppressed nuclear factor (NF)-κB (p65) phosphorylation and caspase 1 activation. Meanwhile, XSLJZD protected TAA-injured liver without increasing fibrosis and enhanced the regulating effect of reversine on RelA, IL-17A, IL-1β, and MCP-1 cytokines. In conclusion, reversine ameliorates liver injury and inhibits inflammation reaction by regulating NF-κB, and XSLJZD protects the liver through its synergistic effect with reversine on regulating inflammatory cytokines.

中文翻译:

Reversine 和中药香沙六君子汤通过调节 RelA/NF-κB/caspase 信号通路改善硫代乙酰胺诱导的肝损伤

摘要 本研究探讨了逆转素和中药香沙六君子汤(XSLJZD)对硫代乙酰胺(TAA)诱导的肝损伤的抗纤维化作用。Sprague-Dawley 大鼠腹膜内给予 TAA,然后腹膜内注射 reversine,和/或口服 XSLJZD。TAA 导致肝损伤,肝脏指数和血清天冬氨酸转氨酶 (AST) 和丙氨酸转氨酶水平升高。Reversine 可减轻肝脏指数和 AST 水平,改善 TAA 诱导的病理变化,但降低 TAA 诱导的胶原沉积、α-平滑肌肌动蛋白和转化生长因子-β1 的表达。Reversine 还调节了炎性细胞因子的 mRNA 水平,例如 RelA、白细胞介素 (IL)-17A、IL-22、IL-1β、IL-6、NLR 家族含有 3、血小板衍生生长因子和单核细胞趋化蛋白,并抑制核因子 (NF)-κB (p65) 磷酸化和半胱天冬酶 1 激活。同时,XSLJZD在不增加纤维化的情况下保护了TAA损伤的肝脏,并增强了逆转素对RelA、IL-17A、IL-1β和MCP-1细胞因子的调节作用。总之,逆转素通过调节NF-κB改善肝损伤并抑制炎症反应,而XSLJZD通过与逆转素协同调节炎症细胞因子来保护肝脏。
更新日期:2020-09-15
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