Streptococcus pyogenes is a known cause of a wide spectrum of diseases, from mild and acute to severe invasive infections. This paper concerns the development of a novel impedimetric biosensor for the detection of the mentioned human pathogen. The proposed biosensor is a gold disk electrode modified with commercially available antibodies attached to the surface of the electrode by carbodiimide chemistry. The conducted tests confirmed the specificity of the antibodies used, which was also demonstrated by the results obtained during the detection of S. pyogenes using electrochemical impedance spectroscopy. The developed sensor successfully detected the presence of S. pyogenes in the sample and the detection limit was calculated as 9.3 cfu/mL. The results obtained show a wide linear range for verified concentrations of this pathogen in a sample from 4.2 × 10² to 4.2 × 10⁶ cfu/mL. Furthermore, the optimal experimentally determined time required to perform pathogen detection in the sample was estimated as 3 min, and the test did not lead to the degradation of the sample.

中文翻译：

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抗体修饰金电极作为化脓性链球菌检测的阻抗生物传感器。

化脓性链球菌是引起各种疾病的已知原因，从轻度和急性到严重的侵袭性感染。本文涉及用于检测所述人类病原体的新型阻抗生物传感器的开发。所提出的生物传感器是金盘电极，其通过碳二亚胺化学修饰有可商购获得的抗体，该抗体附着在电极表面。进行的测试证实了所用抗体的特异性，这也通过使用化学阻抗谱检测化脓性链球菌的过程中获得的结果得到证明。研发的传感器成功检测到化脓性链球菌样品中的总残留量为9.3 cfu / mL。获得的结果表明，该病原体在样品中的验证浓度在4.2×10 ²到4.2×10 ⁶ cfu / mL的宽线性范围内。此外，在样品中进行病原体检测所需的最佳实验确定时间估计为3分钟，并且该测试不会导致样品降解。