Abstract

Calotropis procera produces a milky sap containing proteolytic enzymes. At low concentrations, they induce milk-clotting (60 µg/ml) and to dehair hides (0.05 and 0.1%). A protocol for obtaining the enzymes is reported. The latex was mixed with distilled water and the mixture was cleaned through centrifugation. It was dialyzed with distilled water and centrifuged again to recover the soluble fraction [EP]. The dialyze is a key feature of the process. EP was characterized in terms of protein profile, chemical stability, among other criteria. Wild plants belonging to ten geographic regions and grown in different ecological conditions were used as latex source. Collections were carried out, spaced at three-month, according to the seasons at the site of the study. Proteolytic activity was measured as an internal marker and for determining stability of the samples. EP was also analyzed for metal content and microbiology. EP showed similar magnitude of proteolysis, chromatographic and electrophoretic profiles of proteins. Samples stored at 25 °C exhibited reduced solubility (11%) and proteolytic capacity (11%) after six months. Enzyme autolysis was negligible. Microbiological and metal analyses revealed standard quality of all the samples tested. EP induced milk clotting and hide dehairing after storage for up to six months.

摘要

斜纹夜蛾产生含有蛋白水解酶的乳状汁液。在低浓度下，它们会引起牛奶凝结（60 µg / ml）并导致去毛生皮（0.05和0.1％）。报告了获得酶的方案。将胶乳与蒸馏水混合，并通过离心将混合物清洗。用蒸馏水透析，然后再次离心以回收可溶性级分[EP]。透析是该过程的关键特征。EP通过蛋白质谱，化学稳定性等标准进行表征。属于十个地理区域并在不同生态条件下生长的野生植物被用作乳胶源。根据研究地点的季节，每三个月进行一次收集。测量蛋白水解活性作为内部标记并用于确定样品的稳定性。还对EP的金属含量和微生物学进行了分析。EP表现出相似的蛋白水解，色谱和电泳图谱大小。储存在25°C的样品在六个月后显示出降低的溶解度（11％）和蛋白水解能力（11％）。酶的自溶作用可以忽略不计。微生物和金属分析揭示了所有测试样品的标准质量。EP会导致牛奶凝结，并在储存长达六个月后导致皮毛脱毛。