Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS)-based species identification has found its place in many clinical routine diagnostic laboratories over the past years, allowing significantly reduced turnaround times and high-precision results. With regard to MALDI-TOF MS for filamentous fungi, here, we discuss different approaches for sample processing and growth conditions before analysis. In particular, we review the performances of different commercially available databases as well as the potential of complementary (self-constructed) in-house databases.

中文翻译：

---

关于丝状真菌的MALDI-TOF MS鉴定的更新。

过去几年，基于基质辅助的激光解吸电离飞行时间质谱（MALDI-TOF MS）的物种鉴定已在许多临床常规诊断实验室中占有一席之地，从而大大缩短了周转时间，并提供了高精度结果。关于丝状真菌的MALDI-TOF MS，在这里，我们讨论分析之前样品处理和生长条件的不同方法。特别是，我们回顾了不同商业数据库的性能以及内部数据库的互补性（自行构建）。