Magnetic nanoparticles (MNP) are intensely scrutinized for biomedical applications due to their excellent biocompatibility and adjustable magnetic field (MF) responsiveness. Three‐dimensional spheroid culture of ADSC improves stem cell proliferation and differentiation, increasing their potential for clinical applications. In this study we aimed to detect if MF levitated culture of ADSC loaded with proprietary MNP maintain the properties of ADSC and improve their performances. Levitated ADSC‐MNP formed aggregates with increased volume and reduced number compared to nonlevitated ones. ADSC‐MNP from levitated spheroid displayed higher viability, proliferation and mobility compared to nonlevitated and 2D culture. Levitated and nonlevitated ADSC‐MNP spheroids underwent three lineage differentiation, demonstrating preserved ADSC stemness. Quantitative osteogenesis showed similar values in MNP‐loaded levitated and nonlevitated spheroids. Significant increases in adipogenic conversion was observed for all 3D formulation. Chondrogenic conversion in levitated and nonlevitated spheroids produced comparable ratio glucosaminoglycan (GAG)/DNA. Increased chondrogenesis could be observed for ADSC‐MNP in both levitated and nonlevitated condition. Taken together, ADSC‐MNP levitated spheroids retain stemness and display superior cell viability and migratory capabilities. Furthermore, the method consistently increases spheroid maneuverability, potentially facilitating large scale manufacturing and automation. Levitated spheroid culture of ADSC‐MNP can be further tested for various application in regenerative medicine and organ modeling.

中文翻译：

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悬浮培养中的磁性纳米颗粒负载人脂肪衍生的间充质细胞球体

磁性纳米粒子 (MNP) 因其出色的生物相容性和可调节的磁场 (MF) 响应性而在生物医学应用中受到严格审查。ADSC的三维球体培养提高了干细胞的增殖和分化，增加了它们的临床应用潜力。在这项研究中，我们旨在检测加载专有 MNP 的 MF 悬浮培养物是否保持 ADSC 的特性并改善其性能。与非悬浮的相比，悬浮的 ADSC-MNP 形成的聚集体体积增加，数量减少。与非悬浮和二维培养相比，悬浮球体的 ADSC-MNP 显示出更高的活力、增殖和流动性。悬浮和非悬浮的 ADSC-MNP 球体经历了三个谱系分化，证明了 ADSC 干性的保留。定量成骨在 MNP 加载的悬浮和非悬浮球体中显示出相似的值。观察到所有 3D 配方的脂肪形成转化显着增加。悬浮和非悬浮球体中的软骨形成转化产生了相当比例的葡糖胺聚糖 (GAG)/DNA。在悬浮和非悬浮条件下，ADSC-MNP 的软骨形成增加。总之，ADSC-MNP 悬浮球体保留了干性并显示出卓越的细胞活力和迁移能力。此外，该方法不断提高球体的可操作性，有可能促进大规模制造和自动化。ADSC-MNP 的悬浮球体培养可以进一步测试在再生医学和器官建模中的各种应用。