The ELAV/Hu family is a conserved multigene family of pan‐neuronal RNA‐binding protein involved in post‐transcriptional regulation in metazoans. In Drosophila, three members of this family, ELAV, RBP9 and FNE, are involved in neuronal differentiation, gene expression regulation and so on. This family is less well characterized in Bombyx mori. Two orthologs BmELAV‐like‐1 (BmEL‐1) and BmELAV‐like‐2 (BmEL‐2) share 55%–71% and 47%–62% identity with that of in Drosophila and humans, respectively. Bmel‐1 is ubiquitously expressed while Bmel‐2 is expressed in the head and ovaries specifically. Proteins encoded by both genes are localized in nuclear and cytoplasm. The weight of body, cocoon, pupae and cocoon shell are differently affected in Bmel‐1⁻/−2⁻ mutants created using CRISPR/Cas9 technology. Mutations of both genes increase the expression of four silk protein genes, Fib‐L, Fib‐H, P25 and Ser‐1. In addition, the oviposition ability of Bmel‐2⁻ females is decreased. This study not only provides valuable insights into the functional roles of Bmelav‐like genes in the growth, cocoon characters and regulation of silk protein genes expression, but also provides useful information for silkworm variety breeding.

中文翻译：

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家蚕发育中Bmelav样基因的生物学特性.

ELAV/Hu 家族是泛神经元 RNA 结合蛋白的保守多基因家族，参与后生动物的转录后调控。在果蝇中，该家族的三个成员ELAV、RBP9和FNE参与神经元分化、基因表达调控等。家蚕在家蚕中的特征较少。两个直向同源物 BmELAV-like-1 (BmEL-1) 和 BmELAV-like-2 (BmEL-2)分别与果蝇和人类的同源性有 55%–71% 和 47%–62% 。Bmel-1普遍表达，而Bmel-2专门在头部和卵巢中表达。由这两个基因编码的蛋白质位于细胞核和细胞质中。在使用 CRISPR/Cas9 技术创建的Bmel-1 ^- / -2 ^-突变体中，身体、茧、蛹和茧壳的重量受到不同程度的影响。两种基因的突变都会增加四种丝蛋白基因Fib-L、Fib-H、P25和Ser-1 的表达。此外，产卵能力Bmel-2 ^-女性降低。这项研究不仅为Bmelav 样的功能作用提供了有价值的见解。 基因在生长、茧特性和蚕丝蛋白基因表达的调控，也为蚕品种育种提供了有用的信息。