An 8‐week feeding trial was conducted to investigate the effect of different dietary levels of soybean protein hydrolysates (SPH) on the growth performance, antioxidant capacity, non‐specific immunity and mRNA expression levels of insulin‐like growth factor I (IGF‐I), target of rapamycin (TOR) and oligopeptide transporter 1 (PepT1) for juvenile hybrid grouper (average weight: 12.5 ± 0.40 g). Five iso‐protein and iso‐lipid diets were formulated by adding 0, 10, 20, 40 and 60 g/kg of SPH, respectively. The SPH2‐6 significantly reduced feed conversion ratio and enhanced weight gain rate, specific growth rate and protein efficiency ratio (p < .05). The treatment groups significantly decreased the total protein (p < .05). The cholesterol, low‐density lipoprotein and high‐density lipoprotein of fish fed with SPH6 were significantly lower (p < .05) than control. Fish fed with SPH4 and SPH6 had significantly higher catalase, superoxide dismutase and alkaline phosphatase activities, as well as significantly lower malondialdehyde than the control (p < .05). The levels of aspartate aminotransferase, alanine transaminase, lysozyme, lipase, trypsin, pepsin, complement 3 and complement 4 showed a similar trend of initially increasing and then decreasing. The SPH2‐6 significantly increased IGF‐I and TOR mRNA expressions in liver as well as PepT1 in intestine (p < .05). The optimal SPH inclusion level was estimated to be 42.99 g/kg diet for juvenile hybrid grouper.

中文翻译：

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不同日粮水平大豆蛋白水解物对少年杂交石斑鱼（Epinephelus fuscoguttatus♀×Epinephelus lanceolatus the）生长性能，抗氧化能力和相对mRNA表达水平的影响

进行了为期8周的喂养试验，以研究不同日粮水平的大豆蛋白水解物（SPH）对胰岛素样生长因子I（IGF-I）的生长性能，抗氧化能力，非特异性免疫和mRNA表达水平的影响），雷帕霉素（TOR）和寡肽转运蛋白1（PepT1）的目标，是青少年杂种石斑鱼（平均重量：12.5±0.40 g）。通过分别添加0、10、20、40和60 g / kg的SPH来配制五种同蛋白和异脂饮食。SPH2-6大大降低了饲料转化率，提高了增重率，比生长率和蛋白质效率比（p  <.05）。治疗组的总蛋白显着降低（p <.05）。饲喂SPH6的鱼的胆固醇，低密度脂蛋白和高密度脂蛋白显着低于对照组（p  <.05）。饲喂SPH4和SPH6的鱼的过氧化氢酶，超氧化物歧化酶和碱性磷酸酶活性显着高于对照组，丙二醛也显着低于对照组（p  <.05）。天冬氨酸转氨酶，丙氨酸转氨酶，溶菌酶，脂肪酶，胰蛋白酶，胃蛋白酶，补体3和补体4的水平显示出相似的趋势，即开始增加然后减少。SPH2-6显着增加肝脏以及肠中PepT1的IGF-I和TOR mRNA表达（p  <.05）。对于幼年杂种石斑鱼，最佳SPH含量估计为42.99 g / kg饮食。