Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy ( IF 4.4 ) Pub Date : 2020-09-17 , DOI: 10.1016/j.saa.2020.118953 Beitong Zhu , Xiaoli Wu , João Rodrigues , Xichao Hu , Ruilong Sheng , Guang-Ming Bao
Hydrazine (N2H4) and ClO− are very harmful for public health, hence it is important and necessary to monitor them in living cells. Herein, we rationally designed and synthesized a dual-analytes responsive fluorescent sensor PTMQ for distinguishing detection of N2H4 and ClO−. PTMQ underwent N2H4-induced double bond cleavage, affording colorimetric and green fluorescence enhancement with good selectivity and a low detection limit (89 nM). On the other hand, PTMQ underwent ClO−-induced sulfur oxidation and displayed red fluorescence lighting-up response towards ClO− with good selectivity, rapid response (<0.2 min) and a low detection limit (58 nM). Moreover, PTMQ was successfully employed for in-situ imaging of N2H4 and ClO− in living cells.
中文翻译:
一种双分析物响应性荧光探针用于CLO的判别检测-和N 2 ħ 4在活细胞
肼(N 2 H ^ 4)和CLO -对于公众健康是非常有害的,因此它来监控他们在活细胞是重要和必要的。在此,我们合理设计和合成的双分析物响应性荧光传感器PTMQ用于区分N个检测2 ħ 4和C10 - 。PTMQ经历了N 2 H 4诱导的双键裂解,提供了比色和绿色荧光增强,具有良好的选择性和低检测限(89 nM)。在另一方面,PTMQ行CLO -诱导的硫氧化和显示朝向CLO红色荧光点灯响应-具有很好的选择性,快速反应(<0.2分钟)和低检测限(58纳米)。此外,PTMQ被成功地用于原位N中的成像2 ħ 4和C10 -在活细胞。