A carbon dot (CD)-based fluorometric probe for oxytetracycline (OTC) detection utilizing a Förster resonance energy transfer (FRET) mechanism was firstly developed. Upon addition of OTC, the blue fluorescence of the CDs peaked at 405 nm was quickly quenched, with a new fluorescence peaked at 505 nm attributed to OTC was observed within 30 s. A visual fluorescent color change of the CDs solution from blue to green was discerned under a 365 nm UV light irradiation. The CDs displayed a high sensitivity and selectivity toward OTC with a low detection limit of 0.41 μM. Furthermore, the probe was applied to detect OTC in water, milk, and pork samples with a satisfied recovery.

首次开发了一种基于碳点（CD）的荧光探针，用于利用Förster共振能量转移（FRET）机制检测土霉素。加入OTC后，在405 nm处出现峰的CD的蓝色荧光被快速淬灭，在30 s内观察到归因于OTC的在505 nm处出现峰的新荧光。在365 nm紫外线照射下，可以看到CD溶液从蓝色到绿色的可见荧光颜色变化。CD显示出对OTC的高灵敏度和选择性，低检测限为0.41μM。此外，该探针还用于检测水，牛奶和猪肉样品中的OTC，回收率令人满意。