Canine brucellosis is a contagious disease associated with health implications for humans as well as for a wide range of wild and domesticated animals. In this study, 173 dog blood specimens were sampled from herding dogs in three different provinces including Tehran (n = 127), Qom (n = 40) and Alborz (n = 6) provinces. The presence of Brucella antibodies was determined using Rose Bengal plate test (RBPT), slow agglutination test (SAT) and 2-mercaptoethanol (2-ME), respectively. The seropositive samples were further screened using blood culture and PCR tests to identify and differentiate the implicated Brucella species. According to our results, 24.3% (42/173), 13.8% (24/173) and 6.3% (11/173) of blood samples were tested positive using RBPT, SAT and 2-ME, respectively. However, among 42 seropositive samples, only 38.1% (16/42) and 14.2% (6/42) were positive by PCR and culture, respectively. Brucella melitensis biovar 1 and biovar 2 was isolated from the bacterial cultures of 6 blood samples and confirmed by biotyping, AMOS PCR and Bruce-ladder PCR assays. These findings highlight the potential risk of Brucella transmission from dog to humans along with other livestock and reflect the critical role of infected dogs in the persistence of Brucella infections among ruminant farms. This study stresses the need for further epidemiological investigations on canine brucellosis among herding dogs and suggests the systematic screening of the disease among companion animals such as dogs in order to improve brucellosis surveillance and control programs.

犬布鲁氏菌病是一种传染性疾病，与人类以及各种野生和家养动物的健康有关。在这项研究中，从德黑兰（n = 127），库姆（n = 40）和阿尔伯兹（n = 6）三个省的牧羊犬中采集了173条狗血标本。分别使用Rose Bengal平板试验（RBPT），慢凝集试验（SAT）和2-巯基乙醇（2-ME）确定布鲁氏菌抗体的存在。使用血液培养和PCR测试进一步筛选血清阳性样本，以鉴定和区分所涉及的布鲁氏菌种类。根据我们的结果，分别使用RBPT，SAT和2-ME对血液样本进行了阳性检测，分别为24.3％（42/173），13.8％（24/173）和6.3％（11/173）。但是，在42份血清阳性样本中，分别通过PCR和培养获得阳性的样本分别为38.1％（16/42）和14.2％（6/42）。从6个血样的细菌培养物中分离出布鲁氏布鲁氏菌biovar 1和biovar 2，并通过生物分型，AMOS PCR和Bruce-ladder PCR分析加以确认。这些发现突出了布鲁氏菌与其他牲畜一起从狗传播给人的潜在风险，并反映出感染犬只在布鲁氏菌持续存在中的关键作用。反刍动物农场的感染。这项研究强调需要对成年犬中的犬布鲁氏菌病进行进一步的流行病学调查，并建议对诸如狗等伴侣动物进行系统的疾病筛查，以改善布鲁氏菌病的监测和控制程序。