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Exploration of Anti-plasmodial Activity of Prunus cerasoides Buch.-Ham. ex D. Don (family: Rosaceae) and Its Wood Chromatographic Fractions.
Acta Parasitologica ( IF 1.5 ) Pub Date : 2020-09-17 , DOI: 10.1007/s11686-020-00272-5
Cheryl Sachdeva 1 , Sandeep Kumar 2 , Naveen K Kaushik 1
Affiliation  

Purpose

Increasing resistance to the currently available antimalarial drugs is a leading cause of failure to control malaria. Plant-based medicines are commonly used to manage numerous infections, making medicinal plants the best possible source of alternative antimalarial drugs. The objective of this study is therefore to identify antimalarial potential of Prunus cerasoides.

Methods

Here, anti-plasmodial activity of crude methanolic and aqueous extracts of Prunus cerasoides and fractions obtained by reverse-phase high performance liquid chromatography (RPHPLC) were tested for in vitro activity against chloroquine sensitive Plasmodium falciparum 3D7 and chloroquine resistant INDO & Dd2 strains using SYBR Green I assay. The cytotoxic activity of active extracts/fractions was evaluated against mammalian cell lines-HeLa using MTT assay.

Results

Aqueous extracts of leaves, wood, bark and fruit of P. cerasoides showed poor to no activity up to 100 µg/ml, however methanolic extract showed moderate (IC50: 21–60 µg/ml) to poor (IC50: 61–100 µg/ml) anti-plasmodial activity. Fractionation of wood methanolic extract led to enrichment in antimalarial activity in some of its fractions as out of 17 fractions collected, good anti-plasmodial activity (IC50: 1–20 µg/ml) was shown by three fractions and nine fractions showed moderate anti-plasmodial activity. However, five fractions showed poor to no activity against Plasmodium falciparum (IC50:61–100 and >100 µg/ml respectively). Furthermore, these active fractions showed no cytotoxic effects on mammalian cell lines.

Conclusion

Findings of this study elucidate the anti-plasmodial potential of P. cerasoides and validate its traditional usage suggesting that it could be a possible source of a drug candidate in combating this disease.



中文翻译:

Prunus cerasoides Buch.-Ham 抗疟原虫活性的探索。ex D. Don(家族:蔷薇科)及其木材色谱级分。

目的

对目前可用的抗疟药物的耐药性增加是无法控制疟疾的主要原因。植物性药物通常用于治疗多种感染,使药用植物成为替代抗疟药物的最佳来源。因此,本研究的目的是确定Prunus cerasoides 的抗疟潜力。

方法

在这里,使用 SYBR测试了李属的粗甲醇和水提取物的抗疟原虫活性以及通过反相高效液相色谱 (RPHPLC) 获得的馏分对氯喹敏感的恶性疟原虫3D7 和氯喹抗性 INDO 和 Dd2 菌株的体外活性绿色 I 测定。使用 MTT 测定法针对哺乳动物细胞系-HeLa 评估活性提取物/级分的细胞毒活性。

结果

P. cerasoides的叶子、木材、树皮和果实的水提取物在浓度高达 100 µg/ml 时表现出较差或没有活性,但甲醇提取物的活性中等(IC 50:21–60 µg/ml)至较差(IC 50:61– 100 µg/ml) 抗疟原虫活性。在收集的 17 个馏分中,木材甲醇提取物的分馏导致其某些馏分的抗疟活性增强,三个馏分显示出良好的抗疟原虫活性(IC 50:1–20 µg/ml),九个馏分显示出中等抗疟活性- 疟原虫活性。然而,五个馏分对恶性疟原虫的活性很差甚至没有活性(IC 50:61–100 和 >100 µg/ml)。此外,这些活性组分对哺乳动物细胞系没有细胞毒性作用。

结论

这项研究的结果阐明了P. cerasoides的抗疟原虫潜力,并验证了其传统用途,表明它可能是对抗这种疾病的候选药物的可能来源。

更新日期:2020-09-17
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