Cervical carcinoma is the most common gynaecological cancer in women worldwide. Emerging evidence has shown that long non-coding RNAs (lncRNAs) participate in multiple biological processes of cervical carcinoma tumorigenesis. We aimed to investigate the function of a novel lncRNA RP11-284F21.9 in cervical carcinoma. We found that RP11-284F21.9 was downregulated in cervical carcinoma tissues and cell lines. Overexpression of RP11-284F21.9 inhibits proliferation, invasion and migration of cervical carcinoma cells in vitro. Further, we identified that RP11-284F21.9 directly interacted with miR-769-3p and functioned as the miR-769-3p sponge. Mechanistically, we showed that miR-769-3p regulated PPWD1 expression by targeting PPWD1 3'-UTR. Furthermore, xenograft tumor model revealed that Overexpression of RP11-284F21.9 inhibited tumor growth of cervical carcinoma in vivo. Taken together, our results demonstrate that lncRNA RP11-284F21.9 functions as tumor suppressor and regulates PPWD1 expression through competitively binding to miR-769-3p in cervical carcinoma, suggesting that RP11-284F21.9/miR-769-3p/PPWD1 axis could serve as a promising prognostic biomarker and therapeutic target for cervical carcinoma.

中文翻译：

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长的非编码RNA RP11-284F21.9通过竞争性结合miR-769-3p在宫颈癌中起调控PPWD1的ceRNA的作用。

宫颈癌是全世界女性中最常见的妇科癌症。新兴证据表明，长的非编码RNA（lncRNA）参与子宫颈癌肿瘤发生的多个生物学过程。我们旨在研究新型lncRNA RP11-284F21.9在宫颈癌中的功能。我们发现RP11-284F21.9在宫颈癌组织和细胞系中被下调。RP11-284F21.9的过表达在体外抑制宫颈癌细胞的增殖，侵袭和迁移。此外，我们确定RP11-284F21.9与miR-769-3p直接相互作用，并充当miR-769-3p海绵。从机理上讲，我们表明miR-769-3p通过靶向PPWD1 3'-UTR调节PPWD1表达。此外，异种移植肿瘤模型揭示了RP11-284F21的过表达。9体内抑制宫颈癌的生长。两者合计，我们的结果表明，lncRNA RP11-284F21.9可通过与miR-769-3p竞争性结合而发挥抑癌作用并调节PPWD1的表达，提示RP11-284F21.9 / miR-769-3p / PPWD1轴可以作为宫颈癌的有前途的生物标志物和治疗靶标。