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Thylakoid membrane reorganizations revealed by small-angle neutron scattering of Monstera deliciosa leaves associated with non-photochemical quenching.
Open Biology ( IF 5.8 ) Pub Date : 2020-09-16 , DOI: 10.1098/rsob.200144
Renáta Ünnep 1, 2 , Suman Paul 3 , Ottó Zsiros 4 , László Kovács 4 , Noémi K Székely 5 , Gábor Steinbach 6 , Marie-Sousai Appavou 5 , Lionel Porcar 7 , Alfred R Holzwarth 3 , Győző Garab 4, 8 , Gergely Nagy 2, 9, 10
Affiliation  

Non-photochemical quenching (NPQ) is an important photoprotective mechanism in plants and algae. Although the process is extensively studied, little is known about its relationship with ultrastructural changes of the thylakoid membranes. In order to better understand this relationship, we studied the effects of illumination on the organization of thylakoid membranes in Monstera deliciosa leaves. This evergreen species is known to exhibit very large NPQ and to possess giant grana with dozens of stacked thylakoids. It is thus ideally suited for small-angle neutron scattering measurements (SANS)—a non-invasive technique, which is capable of providing spatially and statistically averaged information on the periodicity of the thylakoid membranes and their rapid reorganizations in vivo. We show that NPQ-inducing illumination causes a strong decrease in the periodic order of granum thylakoid membranes. Development of NPQ and light-induced ultrastructural changes, as well as the relaxation processes, follow similar kinetic patterns. Surprisingly, whereas NPQ is suppressed by diuron, it impedes only the relaxation of the structural changes and not its formation, suggesting that structural changes do not cause but enable NPQ. We also demonstrate that the diminishment of SANS peak does not originate from light-induced redistribution and reorientation of chloroplasts inside the cells.



中文翻译:

与非光化学猝灭相关的龟背竹叶子的小角中子散射揭示了类囊体膜重组。

非光化学猝灭(NPQ)是植物和藻类中重要的光保护机制。尽管该过程已被广泛研究,但对其与类囊体膜超微结构变化的关系知之甚少。为了更好地理解这种关系,我们研究了光照对龟背竹叶子中类囊体膜组织的影响。众所周知,这种常绿物种表现出非常大的 NPQ 并拥有巨大的基粒和数十个堆叠的类囊体。因此,它非常适合小角度中子散射测量(SANS)——一种非侵入性技术,能够提供有关类囊体膜的周期性及其在体内快速重组的空间和统计平均信息。我们发现,NPQ 诱导光照会导致基粒类囊体膜的周期顺序大幅下降。NPQ 的发展和光诱导的超微结构变化以及松弛过程遵循相似的动力学模式。令人惊讶的是,虽然 NPQ 被敌草隆抑制,但它仅阻碍结构变化的松弛,而不阻碍其形成,这表明结构变化不会导致 NPQ,而是会促进 NPQ。我们还证明,SANS 峰值的减弱并非源于光诱导的细胞内叶绿体的重新分布和重新定向。

更新日期:2020-09-16
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