Silk fibroin (SF) is one of the natural biomaterials with promising and growing potential in different clinical applications such as corneal transplantation, donor site skin substitute and tympanic membrane. Some of the SFs that are extracted from mulberry silkworm do not have the arginyl–glycyl–aspartic acid (RGD) sequence for properly supporting cell adhesion and proliferation. Therefore, in the current study, polydopamine (PDA)-coated SFs were prepared to provide an RGD sequence, and the effect of PDA coating on different properties of SF was investigated. The results are also compared with those of an amniotic membrane (AM) that is a commercially available natural biomaterial for the mentioned applications. The Raman spectra showed characteristic peaks at 1581 and 1370 cm⁻¹, which demonstrate the formation of the coating layer on the surface of the films. The results showed that coating led to no significant difference in surface hydrophilicity; a smoother surface; and improved cell attachment and distribution; and a little decrease in membrane transparency, but the membrane still being transparent enough to provide vivid vision through it.

中文翻译：

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贻贝启发的聚多巴胺涂层丝素蛋白作为一种有前途的生物材料

丝素蛋白（SF）是一种天然生物材料，在角膜移植，供体部位皮肤替代品和鼓膜等不同临床应用中具有广阔的发展前景。从桑蚕中提取的一些SF没有精氨酸-甘氨酰-天冬氨酸（RGD）序列，不能正确支持细胞粘附和增殖。因此，在本研究中，制备了聚多巴胺（PDA）涂层的SF，以提供RGD序列，并研究了PDA涂层对SF的不同性能的影响。还将结果与羊膜（AM）进行比较，后者是用于上述应用的可商购天然生物材料。拉曼光谱在1581和1370 cm ^-1处显示特征峰，这表明在薄膜表面上形成了涂层。结果表明，涂​​层不会引起表面亲水性的显着差异。表面较光滑；改善细胞附着和分布；膜的透明度略有下降，但膜仍然足够透明，可以通过它提供生动的视觉效果。