In this study, novel bone substitutes were prepared based on a powder phase composed of tetracalcium phosphate, dicalcium phosphate dihydrate and calcium sulfate dihydrate and a liquid phase composed of carboxymethylcellulose (CMC), citric acid and gelatin. Samples were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD), mechanical testing, swelling–degradation studies and cell culture studies. FTIR results showed that CMC and calcium phosphate interacted through electrostatic forces and hydrogen bonding. After incubation in phosphate-buffered saline for 28 days, hydroxyapatite formation was distinguished by way of SEM and XRD analysis. Mechanical test results revealed that the compressive modulus was up to 1.050 ± 0.071 GPa and that the compressive strength was up to 1.680 ± 0.023 MPa. Cell culture studies indicated that the samples were biocompatible and may be suitable for replacing cancellous bone and supporting new bone formation.

中文翻译：

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磷酸钙/羧甲基纤维素基骨水泥的制备

在这项研究中，基于由磷酸四钙，磷酸二钙二水合物和硫酸钙二水合物组成的粉末相和由羧甲基纤维素（CMC），柠檬酸和明胶组成的液相制备了新型骨替代物。通过傅立叶变换红外光谱（FTIR），扫描电子显微镜（SEM），X射线衍射（XRD），机械测试，溶胀降解研究和细胞培养研究对样品进行表征。FTIR结果表明，CMC和磷酸钙通过静电力和氢键相互作用。在磷酸盐缓冲液中孵育28天后，通过SEM和XRD分析识别了羟基磷灰石的形成。机械测试结果表明，压缩模量高达1.050±0.071 GPa，压缩强度高达1。680±0.023MPa。细胞培养研究表明，样品具有生物相容性，可能适合替代松质骨并支持新的骨形成。