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Cascade signal amplification for sensitive detection of exosomes by integrating tyramide and surface-initiated enzymatic polymerization.
Chemical Communications ( IF 4.9 ) Pub Date : 2020-09-16 , DOI: 10.1039/d0cc04881j
Zhipeng Huang 1 , Qiuyuan Lin 1 , Bin Yang 1 , Xin Ye 1 , Hui Chen 1 , Wenhao Weng 2 , Jilie Kong 1
Affiliation  

A novel cascade signal amplification based on tyramide signal amplification (TSA) and surface-initiated enzymatic polymerization (SIEP) was first reported for the sensitive and template-free detection of colorectal cancer (CRC) exosomes. This assay exhibited 20.9-fold signal amplification with a low detection limit of 12.8 particles per μL. Furthermore, accurate and reproducible results were obtained for detecting exosomes in serum samples, suggesting its potential application in exosomes analysis and clinical diagnostics.

中文翻译:

级联信号放大,通过整合酪酰胺和表面引发的酶促聚合反应灵敏地检测外来体。

首次报道了一种基于酪酰胺信号放大(TSA)和表面引发的酶促聚合(SIEP)的新型级联信号放大,用于大肠癌(CRC)外泌体的灵敏和无模板检测。该测定法显示出20.9倍的信号放大,检测限低至每微升12.8个颗粒。此外,获得了用于检测血清样品中外泌体的准确且可重复的结果,表明其在外泌体分析和临床诊断中的潜在应用。
更新日期:2020-09-23
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