We present a solution to regenerate adipose tissue using degradable, soft, pliable 3D-printed scaffolds made of a medical-grade copolymer coated with polydopamine. The problem today is that while printing, the medical grade copolyesters degrade and the scaffolds become very stiff and brittle, being not optimal for adipose tissue defects. Herein, we have used high molar mass poly(L-lactide-co-trimethylene carbonate) (PLATMC) to engineer scaffolds using a direct extrusion-based 3D printer, the 3D Bioplotter^®. Our approach was first focused on how the printing influences the polymer and scaffold’s mechanical properties, then on exploring different printing designs and, in the end, on assessing surface functionalization. Finite element analysis revealed that scaffold’s mechanical properties vary according to the gradual degradation of the polymer as a consequence of the molar mass decrease during printing. Considering this, we defined optimal printing parameters to minimize material’s degradation and printed scaffolds with different designs. We subsequently functionalized one scaffold design with polydopamine coating and conducted in vitro cell studies. Results showed that polydopamine augmented stem cell proliferation and adipogenic differentiation owing to increased surface hydrophilicity. Thus, the present research show that the medical grade PLATMC based scaffolds are a potential candidate towards the development of implantable, resorbable, medical devices for adipose tissue regeneration.

我们提出了一种使用可降解，柔软，柔韧的3D打印支架再生脂肪组织的解决方案，该支架由涂覆有聚多巴胺的医学级共聚物制成。今天的问题是，在印刷时，医用级共聚酯会降解，并且支架变得非常僵硬，易碎，对于脂肪组织缺损不是最佳的选择。在本文中，我们已经使用高摩尔质量的聚（L-丙交酯-共-三亚甲基碳酸酯）（PLATMC）来设计使用直接基于挤压的三维打印机，该三维Bioplotter支架^®。我们的方法首先关注印刷如何影响聚合物和支架的机械性能，然后研究不同的印刷设计，最后评估表面功能。有限元分析表明，由于印刷过程中摩尔质量的降低，脚手架的机械性能根据聚合物的逐渐降解而变化。考虑到这一点，我们定义了最佳的印刷参数，以最大程度地减少材料的降解以及采用不同设计的印刷脚手架。我们随后使用聚多巴胺涂层功能化了一种支架设计，并进行了体外细胞研究。结果表明，由于表面亲水性增加，聚多巴胺增强了干细胞的增殖和成脂分化。从而，