Due to the unavailability of any specific countermeasure, the constantly spreading COVID-19 pandemic could only be partially and temporarily slowed down by implementing regional lockdowns that force people to stay at home and prevent their movement. With the progression of the pandemic, a considerable subset of the population would have acquired post-infection immunity and the tests that reveal the post-infection immune status of individuals are the need of the hour. A credible test, which accurately identifies the protected, can offer an immunity passport for the individual to be freed from the lockdown and resume routine activities without the fear of getting infected. At present, the semi-quantitative neutralization test (NT) and the quantitative plaque reduction neutralization test (PRNT) identifying the presence of anti-SARS-CoV-2 neutralizing antibodies (nAbs) are the only foolproof methods available for this purpose. However, practical feasibility of these highly specific tests is weighed down by drawbacks such as low throughput, long turnaround time (TAT), and the need for a specialized laboratory setup with biosafety level 3 (BSL3) facilities to handle the live viruses used in these tests (Cao et al., 2020).

中文翻译：

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使用HiBiT标签的病毒样颗粒对SARS-CoV-2中和抗体进行快速定量筛选测定

由于没有任何具体的对策，只能通过实施区域封锁来迫使人们呆在家里并阻止其行动，从而部分和暂时地减缓不断传播的COVID-19大流行。随着大流行的发展，相当一部分人口将获得感染后免疫力，而揭示个体感染后免疫状态的测试是一个小时的需要。可以准确识别受保护者的可信测试可以为个人提供免税护照，使他们从锁定中解脱出来并恢复日常活动，而不必担心被感染。现在，鉴定抗SARS-CoV-2中和抗体（nAbs）存在的半定量中和试验（NT）和斑块减少中和试验（PRNT）是唯一可用于此目的的简便方法。但是，这些高特异性测试的实际可行性受到诸如低通量，较长的周转时间（TAT）以及需要具有生物安全3级（BSL3）设施的专门实验室设置以处理其中使用的活病毒之类的缺点的影响。测试（Cao et al。，2020）。