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Development of a Physiological Age-Grading System for Nezara viridula (Hemiptera: Pentatomidae).
Journal of Insect Science ( IF 2.2 ) Pub Date : 2020-09-16 , DOI: 10.1093/jisesa/ieaa092
Michael J Grodowitz 1 , Brad Elliot 2 , Richard Evans 1 , Guadalupe Rojas 1 , Juan Morales Ramos 1
Affiliation  

The southern green stink bug (SGSB), Nezara viridula (L.), is an important agricultural pest in the United States. Limited information is available on the morphology of the female’s reproductive system in relation to morphological changes associated with the number of eggs produced and egg masses oviposited. The ability to assess reproductive health and reproductive status based on ovarian morphology (i.e., physiological age-grading) can be an important tool for evaluating field populations and laboratory colonies intended for the application of different management strategies and experimental trials. Thus, the goal of this study was to develop a physiological age-grading system for SGSB. Females aged from 0 to 79 d chronologically randomly selected from laboratory colonies and dissected to assess ovarian morphology. Specific morphological differences in ovarian structures including differentiation of the ovarioles, deposition of yolk in the most proximal follicle, quantity and appearance of follicular relics, expansion of the lateral oviducts, and number of developing follicles per ovariole were related to chronological age, the number of eggs produced and number of egg masses. Based on specific combinations of these morphological characteristics, the continuum of ovarian development was divided into three nulliparous (i.e., ‘no eggs’; N1, N2, and N3) and three parous stages (i.e., ‘with eggs’; P1, P2, and P3). Direct relationships were noted between number of eggs produced and physiological age with over 7-fold higher number of eggs and 14-fold higher number of egg masses associated with the P2 and P3 stages, respectively.

中文翻译:

内扎斑鸠草(半翅目:昆虫科)的生理年龄分级系统的开发。

南方绿蝽(上工申贝),稻绿蝽(L.),是美国重要的农业害虫。关于雌性生殖系统形态的有限信息,与产卵数和产卵量有关的形态变化有关。基于卵巢形态学(即生理年龄分级)评估生殖健康和生殖状况的能力可能是评估野外种群和实验室菌落的重要工具,旨在应用不同的管理策略和实验研究。因此,本研究的目的是为SGSB开发生理年龄分级系统。从实验室菌落按时间顺序随机选择0至79 d的雌性,并解剖以评估卵巢形态。卵巢结构的特定形态学差异包括卵巢的分化,卵泡在最近端卵泡的沉积,卵泡遗物的数量和外观,侧输卵管的扩张以及每个卵巢的发育中的卵泡数目与年龄,年龄和年龄有关。产蛋量和蛋重数。根据这些形态特征的特定组合,将卵巢发育的连续体分为三个卵生期(即“无卵”; N1,N2和N3)和三个卵生期(即“有卵”; P1,P2,和P3)。注意到产卵数与生理年龄之间存在直接关系,与P2和P3期相关的卵数分别高出7倍和14倍。卵黄在最接近的卵泡中的沉积,卵泡遗物的数量和外观,侧输卵管的扩张以及每个卵子发育的卵泡的数量与年代,年龄,产卵数和卵量有关。根据这些形态特征的特定组合,将卵巢发育的连续体分为三个卵生期(即“无卵”; N1,N2和N3)和三个卵生期(即“有卵”; P1,P2,和P3)。注意到产卵数与生理年龄之间存在直接关系,分别与P2和P3阶段相关的卵数高出7倍,卵块数高出14倍。卵黄在最接近的卵泡中的沉积,卵泡遗物的数量和外观,侧输卵管的扩张以及每个卵子发育的卵泡的数量与年代,年龄,产卵数和卵量有关。根据这些形态特征的特定组合,将卵巢发育的连续体分为三个卵生期(即“无卵”; N1,N2和N3)和三个卵生期(即“有卵”; P1,P2,和P3)。注意到产卵数与生理年龄之间存在直接关系,与P2和P3期相关的卵数分别高出7倍和14倍。每个卵巢的卵泡发育和卵泡数量与年龄,卵产卵数和卵重有关。根据这些形态特征的特定组合,将卵巢发育的连续体分为三个卵生期(即“无卵”; N1,N2和N3)和三个卵生期(即“有卵”; P1,P2,和P3)。注意到产卵数与生理年龄之间存在直接关系,与P2和P3期相关的卵数分别高出7倍和14倍。每个卵巢的卵泡发育,卵泡数量与年龄,卵产卵数和卵重有关。根据这些形态特征的特定组合,将卵巢发育的连续体分为三个卵生期(即“无卵”; N1,N2和N3)和三个卵生期(即“有卵”; P1,P2,和P3)。注意到产卵数与生理年龄之间存在直接关系,与P2和P3期相关的卵数分别高出7倍和14倍。“没有鸡蛋”;N1,N2和N3)和三个偶发阶段(即“带卵”; P1,P2和P3)。注意到产卵数与生理年龄之间存在直接关系,分别与P2和P3阶段相关的卵数高出7倍,卵块数高出14倍。“没有鸡蛋”;N1,N2和N3)和三个偶发阶段(即“带卵”; P1,P2和P3)。注意到产卵数与生理年龄之间存在直接关系,与P2和P3期相关的卵数分别高出7倍和14倍。
更新日期:2020-09-16
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