Abstract Introduction This study determined the presence of nitric oxide synthesis isoforms (nNOS, iNOS, and eNOS) in thoracic spinal cord segments and nodose ganglia of rats with gamma-irradiated livers. Material and Methods Male rats (n = 32) were divided into equal groups A, B, C, and D. In group A, the controls, no radiation was applied, while groups B, C, and D received 10 Gy of ionising gamma radiation. The rats of group B were euthanized at the end of the first day (d1), those of group C on the second day (d2), and those of group D on the third day (d3). The liver, spinal cord segments, and nodose ganglion tissues were dissected and fixed, and the liver sections were examined histopathologically. The other tissues were observed through a light microscope. Results Regeneration occurred at the end of d3 in hepatocytes which were radiation-damaged at the end of d1 and d2. On d1, some nNOS-positive staining was found in the neuronal cells of laminae I–III of the spinal cord and in neurons of the nodose ganglion, and on d3, some staining was observed in lamina X of the spinal cord, while none of note was in the nodose ganglion. Dense iNOS-positive staining was seen on d1 in the ependymal cells of the spinal cord and in the glial cells of the nodose ganglion, and on d3, there was still considerable iNOS staining in both tissues. There was clear eNOS-positive staining in the capillary endothelial cells of the spinal cord and light diffuse cytoplasmic staining in the neurons of the nodose ganglion on d1, and on d3, intense eNOS-positive staining was visible in several endothelial cells of the spinal cord, while light nuclear staining was recognised in the neurons of the nodose ganglion. Conclusion The nNOS, iNOS, and eNOS isoforms are activated in the spinal cord and nodose ganglion of rats after ionising radiation insult to the liver.

中文翻译：

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对接受电离 γ 辐射到其肝脏的大鼠的脊髓和结节神经节中一氧化氮合酶同种型 (nNOS、iNOS、eNOS) 的免疫组织化学研究

摘要 简介 本研究确定了一氧化氮合成异构体（nNOS、iNOS 和 eNOS）在受到伽马射线照射的肝脏大鼠的胸段脊髓节段和结节神经节中的存在。材料和方法 雄性大鼠（n = 32）被分成相等的 A、B、C 和 D 组。A 组为对照组，不进行辐射，而 B、C 和 D 组接受 10 Gy 电离伽马辐射。B组第1天(d1)结束安乐死，C组第2天(d2)安乐死，D组第3天(d3)。解剖并固定肝脏、脊髓节段和结节神经节组织，并对肝脏切片进行组织病理学检查。通过光学显微镜观察其他组织。结果 d1和d2末辐射损伤的肝细胞在d3末发生再生。d1时，脊髓I-III层神经元细胞和结节神经元中发现一些nNOS阳性染色，d3时，脊髓X层中观察到一些nNOS染色，而没有一个注意在结节神经节。d1脊髓室管膜细胞和结节神经节神经胶质细胞中iNOS呈密集阳性染色，d3时两种组织仍有大量iNOS染色。d1脊髓毛细血管内皮细胞eNOS阳性染色明显，结节神经元胞浆轻度弥漫性染色，d3脊髓多处内皮细胞可见eNOS强阳性染色, 而在结节神经节的神经元中发现了轻微的核染色。结论 电离辐射损伤肝脏后大鼠脊髓和结节神经节中的nNOS、iNOS和eNOS亚型被激活。