Abstract Introduction The article describes the occurrence and phylogenetic relationship of Salmonella isolates found in subcutaneous abscesses of leopard geckos. The aim of the study was to determine the cause of the abscesses and to characterise isolated Salmonella strains. Material and Methods Samples of abscesses from five animals and internal organs (lungs, liver, and gut) of three of them were tested for Salmonella according to the PN-EN ISO 6579:2002/A1:2007 standard. The antimicrobial resistance was evaluated by minimal inhibitory concentrations and the genetic similarity of the isolates was assessed with pulsed field gel electrophoresis (PFGE). Results In total, seventeen Salmonella isolates belonging to five different serovars were found to be susceptible to all tested antimicrobials except streptomycin. The serovars were S. Hadar, S. Fluntern, S. Tennessee, S. enterica subsp. salamae 55:k:z39, and S. Kentucky. Up to three serovars from different organs were isolated from the same individual. In two geckos, Salmonella were detected in the lungs. In three serovars, XbaI-PFGE typing revealed indistinguishable isolates from organs and abscesses. Conclusion Multiple Salmonella serovars might be involved in abscess formation and infections. The occurrence of the same PFGE profiles of the isolates may testify to the role of opportunistic organisms in causing infection.

中文翻译：

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豹纹壁虎（Eublepharis macularius）皮下脓肿中发现的沙门氏菌分离株的遗传关系

摘要 引言 本文介绍了在豹纹壁虎皮下脓肿中发现的沙门氏菌分离株的发生情况和系统发育关系。该研究的目的是确定脓肿的原因并鉴定分离的沙门氏菌菌株。材料和方法 根据 PN-EN ISO 6579:2002/A1:2007 标准，对来自五只动物和其中三只内脏器官（肺、肝和肠）的脓肿样本进行沙门氏菌检测。通过最小抑制浓度评估抗菌素耐药性，并通过脉冲场凝胶电泳（PFGE）评估分离物的遗传相似性。结果总共发现属于 5 种不同血清型的 17 种沙门氏菌分离株对除链霉素外的所有测试抗菌剂均敏感。血清型是 S. Hadar、S. Fluntern, S. Tennessee, S. enterica subsp。salamae 55:k:z39 和 S. Kentucky。从同一个体中分离出多达三种来自不同器官的血清型。在两只壁虎的肺部检测到沙门氏菌。在三个血清型中，XbaI-PFGE 分型显示与器官和脓肿无法区分的分离物。结论 多种沙门氏菌血清型可能与脓肿形成和感染有关。分离株的相同 PFGE 谱的出现可能证明机会性微生物在引起感染中的作用。结论 多种沙门氏菌血清型可能与脓肿形成和感染有关。分离株的相同 PFGE 谱的出现可能证明机会性微生物在引起感染中的作用。结论 多种沙门氏菌血清型可能与脓肿形成和感染有关。分离株的相同 PFGE 谱的出现可能证明机会性微生物在引起感染中的作用。