Abstract With environmental health and safety becoming a concern for people, supercritical fluid chromatography has been regarded as a “green chemistry” method that gradually has peaked people’s interest. The purpose of this study was to establish a method to screen and quantify the quality markers of Duhuo (DH) through an environmentally friendly ultra-performance convergence chromatography (UPC2) method. Firstly, the fingerprint of different batches of DH samples was established by UPC2. Then, the quality markers that can represent the whole information of DH was screened through chemometrics. Finally, the content of the selected quality markers was determined using the UPC2 method. From the fingerprint analysis, 24 common peaks were identified. The quantitative analysis results showed that the four compounds could be used as quality markers to represent the overall information and to monitor the quality of DH. This is the first time a traditional Chinese medicine (TCM) fingerprint has been established by UPC2 and also the first time to screen the quality markers in DH. The method established in this study can be used for a comprehensive and overall analysis of DH or other natural medicines, and it could serve as an efficient method to contribute to the expansion of the global market for TCMs. Graphical Abstract

中文翻译：

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基于指纹建模的独活品质标志物的筛选与量化

摘要 随着环境健康与安全日益受到人们的关注，超临界流体色谱作为一种“绿色化学”方法逐渐受到人们的关注。本研究的目的是建立一种通过环保的超高效合相色谱（UPC2）方法筛选和量化独活（DH）质量标志物的方法。首先通过UPC2建立不同批次DH样品的指纹图谱。然后，通过化学计量学筛选出能够代表DH全部信息的质量标志物。最后，使用 UPC2 方法确定所选质量标记的含量。从指纹分析中，确定了 24 个常见峰。定量分析结果表明，这四种化合物可作为质量标志物，代表整体信息并监测DH的质量。这是UPC2首次建立中药指纹图谱，也是首次对DH中的质量标志物进行筛选。本研究建立的方法可用于对 DH 或其他天然药物进行全面、全面的分析，可以作为一种有效的方法为扩大全球中药市场做出贡献。图形概要 本研究建立的方法可用于对 DH 或其他天然药物进行全面、全面的分析，可以作为一种有效的方法为扩大全球中药市场做出贡献。图形概要 本研究建立的方法可用于对 DH 或其他天然药物进行全面、全面的分析，可以作为一种有效的方法为扩大全球中药市场做出贡献。图形概要