Despite the role of pigs as a source of new Influenza A Virus viruses (IAV) potentially capable of initiating human pandemics, immune responses to swine influenza virus (SwIV) in pigs are not fully understood. Several SwIV epitopes presented by swine MHC (SLA) class I have been identified using different approaches either in outbred pigs or in Babraham large white inbred pigs, which are 85% identical by genome wide SNP analysis. On the other hand, some class II SLA epitopes were recently described in outbred pigs. In this work, Babraham large white inbred pigs were selected to identify SLA II epitopes from SwIV H1N1. PBMCs were screened for recognition of overlapping peptides covering the NP and M1 proteins from heterologous IAV H1N1 in IFNγ ELISPOT. A novel SLA class II restricted epitope was identified in NP from swine H1N1. This conserved novel epitope could be the base for further vaccine approaches against H1N1 in pigs.

尽管猪在潜在地引发人间大流行的新型甲型流感病毒（IAV）中扮演着角色，但对猪中猪流感病毒（SwIV）的免疫反应仍未完全了解。猪MHC（ILA）I类所呈现的几种SwIV表位已在异种猪或Babraham大型白色近交猪中使用不同的方法进行了鉴定，通过全基因组SNP分析，它们具有85％的相同性。另一方面，近来在近亲猪中描述了一些II类SLA表位。在这项工作中，选择了Babraham大的白色近交猪来从SwIV H1N1中鉴定SLA II表位。筛选PBMC以识别覆盖IFNγELISPOT中来自异源IAV H1N1的NP和M1蛋白的重叠肽。从猪H1N1的NP中鉴定出新的SLA II类限制性表位。