Systemic signaling and systemic acquired acclimation (SAA) are essential for plant survival during episodes of environmental stress. Recent studies highlighted a key role for reactive oxygen species (ROS) signaling in mediating systemic responses and SAA during light stress in Arabidopsis (Arabidopsis thaliana). These studies further identified the RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD) protein as a key player in mediating rapid systemic ROS responses. Here, we report that tissue-specific expression of RBOHD in phloem or xylem parenchyma cells of the rbohD mutant restores systemic ROS signaling, systemic stress-response transcript expression, and SAA to a local treatment of light stress. We further demonstrate that RBOHD and RBOHF are both required for local and systemic ROS signaling at the vascular bundles of Arabidopsis. Taken together, our findings highlight a key role for RBOHD-driven ROS production at the vascular bundles of Arabidopsis in mediating light stress–induced systemic signaling and SAA. In addition, they suggest that the integration of ROS, calcium, electric, and hydraulic signals, during systemic signaling, occurs at the vascular bundles.

系统信号传导和系统获得性驯化（SAA）对于植物在环境胁迫期间的生存至关重要。最近的研究强调了活性氧 (ROS) 信号在拟南芥 ( Arabidopsis thaliana )光胁迫期间介导系统反应和 SAA 中的关键作用。这些研究进一步确定了呼吸爆发氧化酶同源 D (RBOHD) 蛋白是介导快速全身 ROS 反应的关键参与者。在这里，我们报告rbohD突变体的韧皮部或木质部薄壁细胞中RBOHD的组织特异性表达恢复了系统ROS信号传导、系统应激反应转录本表达和SAA以局部治疗光应激。我们进一步证明RBOHD和RBOHF是拟南芥维管束局部和全身 ROS 信号传导所必需的。总而言之，我们的研究结果强调了拟南芥维管束中 RBOHD 驱动的 ROS 产生在介导光应激诱导的系统信号传导和 SAA 中的关键作用。此外，他们认为，在全身信号传导过程中，ROS、钙、电和液压信号的整合发生在维管束处。