Salmonella and Shigella genera are common pathogens that contaminate foods and beverages. Lateral flow assays (LFA) are commonly used to detect these pathogens. However, most of the developed LFAs are for single detection. Simultaneous detection of pathogens is required to reduce cost and time. In this work, 40 nm gold nanoparticles (AuNPs) were synthesized using the seeding growth method as labeling agent. The AuNPs were characterized and conjugated with mouse anti-Gram negative endotoxin antibody. The nitrocellulose membrane HF135 was immobilized with anti-mouse IgG antibody as a control line and two separate test lines with either anti-Shigella or anti-Salmonella antibody, respectively. Color intensity of test lines was observed for positive samples. A milk sample was used as proof of concept to mimic actual contamination. The limit of detection of the LFA was 3.0 × 10⁶ CFU/mL for multiplex detection of Shigella flexneri and Salmonella Typhi and for both single detections. The result was comparable with the enzyme-linked immunosorbent assay (ELISA) analysis. The produced LFA could differentiate between Shigella flexneri, Shigella boydii, Salmonella Enteritidis, and Salmonella Typhi. The developed LFA was able to identify Shigella flexneri and Salmonella Typhi with good sensitivity in milk samples, thus, beneficial to ensure the safety of food before entering the market.

中文翻译：

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开发基于金纳米粒子的快速侧流分析，用于同时检测志贺氏菌和沙门氏菌属

沙门氏菌和志贺氏菌属是污染食品和饮料的常见病原体。侧向流分析 (LFA) 通常用于检测这些病原体。然而，大多数开发的 LFA 都是用于单次检测的。需要同时检测病原体以减少成本和时间。在这项工作中，使用种子生长法作为标记剂合成了 40 nm 金纳米粒子 (AuNPs)。AuNPs 被表征并与小鼠抗革兰氏阴性内毒素抗体偶联。硝酸纤维素膜 HF135 固定有抗小鼠 IgG 抗体作为对照线和两条单独的测试线，带有抗志贺氏菌或抗沙门氏菌抗体，分别。对于阳性样品，观察测试线的颜色强度。牛奶样品被用作模拟实际污染的概念证明。LFA 的检测限为 3.0 × 10 ⁶ CFU/mL，用于弗氏志贺氏菌和伤寒沙门氏菌的多重检测以及两种单一检测。结果与酶联免疫吸附测定 (ELISA) 分析相当。生产的 LFA 可以区分志贺氏志贺菌、博氏志贺菌、肠炎沙门氏菌和伤寒沙门氏菌。开发的 LFA 能够识别志贺氏菌和沙门氏菌 伤寒菌在牛奶样品中具有良好的灵敏度，有利于确保食品进入市场前的安全。