Abstract To comprehensively evaluate the impact of 2450 MHz electromagnetic wave (EW) loading on returned sludge (RS) for sludge reduction and pollutants removal in anaerobic/anoxic/oxic (A2O) process, the performance of the coupled process (EWRS-A2O) was investigated in current study. 250 W was the appropriate loading power when considering both biological effect and dissolution effect of electromagnetic wave on the returned sludge. After being loaded, floc structure of the returned sludge became looser, and the microbial composition changed. The continuous operation indicated that an 8.3 % improvement in total nitrogen removal and a 33.1 % of sludge reduction were achieved in EWRS-A2O, accompanied by an observed yield coefficient of 0.35 kg SS/kg COD. Although an increase in the total phosphorus concentration of the effluent was found, the impacts on the chemical oxygen demand and ammonia nitrogen of the effluent were minimal. When contrasted to conventional A2O, EWRS-A2O process exhibited higher microbial metabolic activity. Miseq sequencing results showed that the microbial richness increased in EWRS-A2O system with changing of the microbial community structure. Additionally, functional microorganism analysis revealed the enrichment of slow-growing bacteria (Dechloromonas), fermenting bacteria (Clostridium sensu stricto and Tolumonas), and heterotrophic denitrifying bacteria (Thermomonas).

中文翻译：

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2450 MHz电磁波加载回流污泥厌氧-缺氧-好氧反应器的污泥减量和污染物去除：性能与机理

摘要 为了综合评价 2450 MHz 电磁波 (EW) 加载对回流污泥 (RS) 对厌氧/缺氧/好氧 (A2O) 过程中污泥减量和污染物去除的影响，耦合过程 (EWRS-A2O) 的性能是在目前的研究中进行了调查。考虑到电磁波对回流污泥的生物效应和溶解效应，250 W为合适的加载功率。装载后，回流污泥的絮体结构变得松散，微生物组成发生变化。连续运行表明，在 EWRS-A2O 中，总氮去除率提高了 8.3%，污泥减少了 33.1%，同时观察到的产量系数为 0.35 kg SS/kg COD。虽然发现出水总磷浓度有所增加，对出水化学需氧量和氨氮的影响很小。与传统的 A2O 相比，EWRS-A2O 工艺表现出更高的微生物代谢活性。Miseq测序结果表明，EWRS-A2O系统中微生物丰富度随着微生物群落结构的变化而增加。此外，功能微生物分析揭示了生长缓慢的细菌（脱氯单胞菌）、发酵细菌（严格梭状芽孢杆菌和 Tolumonas）和异养反硝化细菌（热单胞菌）的富集。Miseq测序结果表明，EWRS-A2O系统中微生物丰富度随着微生物群落结构的变化而增加。此外，功能微生物分析揭示了生长缓慢的细菌（脱氯单胞菌）、发酵细菌（严格梭状芽孢杆菌和 Tolumonas）和异养反硝化细菌（热单胞菌）的富集。Miseq测序结果表明，EWRS-A2O系统中微生物丰富度随着微生物群落结构的变化而增加。此外，功能微生物分析揭示了生长缓慢的细菌（脱氯单胞菌）、发酵细菌（严格梭状芽孢杆菌和 Tolumonas）和异养反硝化细菌（热单胞菌）的富集。