Abstract The development of an effective delivery vehicle that gives rise to a microenvironment that promotes skin regeneration remains a challenge in the field of stem cell-based therapeutic strategy for skin tissue engineering. Here, we fabricated a photo-crosslinkable hydrogel based on a decellularized dermal matrix (DDM) combined with o-nitrobenzene-modified hyaluronic acid (HA-NB). The HA-NB/DDM hydrogels can be crosslinked in situ under UV ultraviolet light irradiation and adhere strongly to the surrounding skin tissue. Results showed that nude mice treated with the HA-NB/DDM hydrogel loaded with adipose-derived stem cells (ASCs) accelerated wound healing compared with those treated with the hydrogel or ASCs alone and untreated control animals. Neo-epidermal formation and angiogenesis was better in wounds treated with ASC-loaded hydrogels. Further, epidermal–dermal junctions with healthy epidermal protrusions and dermal projections were observed in wounds treated with the ASC-loaded HA-NB/DDM hydrogels. Collagen deposits resembling the natural collagen structure and arrangement were observed in wounds treated with the ASC-loaded HA-NB/DDM hydrogel. Further, ASC-loaded HA-NB/DDM hydrogels can achieve satisfactory therapeutic effects in wounds with irregular defects. Hence, the developed HA-NB/DDM hydrogel can serve as a stem-cell delivery platform that undergoes rapid in situ gelation and strong adhesion with the surrounding tissue and accelerate wound healing.

中文翻译：

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基于脱细胞真皮基质的光交联水凝胶作为递送脂肪干细胞以加速皮肤伤口愈合的平台

摘要 开发一种能产生促进皮肤再生的微环境的有效递送载体仍然是基于干细胞的皮肤组织工程治疗策略领域的挑战。在这里，我们制造了一种基于脱细胞真皮基质 (DDM) 与邻硝基苯改性透明质酸 (HA-NB) 的光交联水凝胶。HA-NB/DDM 水凝胶可以在紫外线紫外线照射下原位交联，并牢固地粘附在周围的皮肤组织上。结果表明，与单独使用水凝胶或 ASCs 处理的裸鼠和未处理的对照动物相比，用装载有脂肪干细胞 (ASC) 的 HA-NB/DDM 水凝胶处理的裸鼠加速了伤口愈合。新表皮形成和血管生成在用负载 ASC 的水凝胶治疗的伤口中更好。此外，在用负载 ASC 的 HA-NB/DDM 水凝胶治疗的伤口中观察到具有健康表皮突起和真皮突起的表皮 - 真皮连接。在用载有 ASC 的 HA-NB/DDM 水凝胶处理的伤口中观察到类似于天然胶原结构和排列的胶原沉积。此外，负载 ASC 的 HA-NB/DDM 水凝胶可以在不规则缺损的伤口中取得令人满意的治疗效果。因此，开发的 HA-NB/DDM 水凝胶可以作为干细胞递送平台，进行快速原位凝胶化和与周围组织的强粘附并加速伤口愈合。在用载有 ASC 的 HA-NB/DDM 水凝胶处理的伤口中观察到类似于天然胶原结构和排列的胶原沉积。此外，负载 ASC 的 HA-NB/DDM 水凝胶可以在不规则缺损的伤口中取得令人满意的治疗效果。因此，开发的 HA-NB/DDM 水凝胶可以作为干细胞递送平台，进行快速原位凝胶化和与周围组织的强粘附并加速伤口愈合。在用载有 ASC 的 HA-NB/DDM 水凝胶处理的伤口中观察到类似于天然胶原结构和排列的胶原沉积。此外，负载 ASC 的 HA-NB/DDM 水凝胶可以在不规则缺损的伤口中取得令人满意的治疗效果。因此，开发的 HA-NB/DDM 水凝胶可以作为干细胞递送平台，进行快速原位凝胶化和与周围组织的强粘附并加速伤口愈合。