The removal of lignin is important to the recovery of saccharides from the pre-hydrolysis liquor (PHL) in kraft-based dissolved pulp production. A one-step process for lignin removal from PHL via treatment with horseradish peroxidase (HRP) in the presence of Ca²⁺ was proposed, and its principle was studied. The results demonstrated synergy between HRP and Ca²⁺ in lignin removal from PHL, whereas NH₄⁺ had little effect on lignin removal. HRP treatment in the presence of 60 mmol/L of Ca²⁺ resulted in a lignin removal of 64.8% accompanied by a saccharide loss of 14.2%. HRP catalyzed both the polymerization and depolymerization of the lignin in the PHL. The HRP-catalyzed lignin polymerization rendered some lignin insoluble enabling it to be directly removed. The HRP-catalyzed depolymerization of lignin decreased its molecular weight with an evident increase in its carboxyl content. The insoluble complexes formed between the lignin with carboxyl and the Ca²⁺ facilitated the removal of the depolymerized lignin.

木质素的去除对于在基于牛皮纸的溶解纸浆生产中从预水解液（PHL）中回收糖类很重要。提出了一种在Ca ²⁺存在下用辣根过氧化物酶（HRP）处理从PHL中去除木质素的一步法，并对其原理进行了研究。结果表明，HRP和Ca ²⁺在去除PHL中的木质素方面具有协同作用，而NH ₄ ⁺对去除木质素的影响很小。在60 mmol / L Ca ²⁺存在下进行HRP处理导致木质素去除率为64.8％，糖损失为14.2％。HRP催化了PHL中木质素的聚合和解聚。HRP催化的木质素聚合反应使某些木质素不溶，使其可以直接除去。HRP催化的木质素解聚反应降低了其分子量，并明显增加了其羧基含量。木质素与羧基和Ca ²⁺之间形成的不溶性配合物促进了解聚木质素的去除。