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Transcriptome analysis reveals the genetic basis of skeletal muscle glycolytic potential based on a pig model.
Gene ( IF 3.5 ) Pub Date : 2020-09-16 , DOI: 10.1016/j.gene.2020.145157
Wangjun Wu 1 , Zengkai Zhang 1 , Zhe Chao 2 , Bojiang Li 1 , Rongyang Li 1 , Aiwen Jiang 1 , Kee-Hong Kim 3 , Honglin Liu 1
Affiliation  

Glycolytic potential (GP) calculated based on glucose, glycogen, glucose-6-phosphate, and lactate contents is a critical factor for multiple meat quality characteristics. However, the genetic basis of glycolytic metabolism is still unclear. In this study, we constructed six RNA-Seq libraries using longissimus dorsi (LD) muscles from pigs divergent for GP phenotypic values and generated the whole genome-wide gene expression profiles. Furthermore, we identified 25,880 known and 220 novel genes from these skeletal muscle libraries, and 222 differentially expressed genes (DEGs) between the higher and lower GP groups. Notably, we found that the Lactate dehydrogenase B (LDHB) and Fructose-2, 6-biphosphatase 3 (PFKFB3) expression levels were higher in the higher GP group than the lower GP group, and positively correlated with GP and lactic acid (LA), and reversely correlated with pH value at 45 min postmortem (pH45min). Besides, LDHB and PFKFB3 expression were positively correlated with drip loss measured at 48 h postmortem (DL48h) and drip loss measured at 24 h postmortem (DL24h). Collectively, we identified a serial of DEGs as the potential key candidate genes affecting GP and found that LDHB and PFKFB3 are closely related to GP and GP-related traits. Our results lay a solid basis for in-depth studies of the regulatory mechanisms on GP and GP-related traits in pigs.



中文翻译:

转录组分析揭示了基于猪模型的骨骼肌糖酵解潜力的遗传基础。

基于葡萄糖,糖原,6-磷酸葡萄糖和乳酸含量计算出的糖酵解潜能(GP)是多种肉品质特征的关键因素。但是,糖酵解代谢的遗传基础仍不清楚。在这项研究中,我们使用猪背最长肌(LD)肌肉构建GP表型值,构建了六个RNA-Seq文库,并生成了全基因组基因表达谱。此外,我们从这些骨骼肌文库中鉴定了25,880个已知基因和220个新基因,以及在较高GP组和较低GP组之间的222个差异表达基因(DEG)。值得注意的是,我们发现乳酸脱氢酶BLDHB)和果糖2、6-双磷酸酶3PFKFB3)高GP组的表达水平高于低GP组,并与GP和乳酸(LA)正相关,而与死后45min(pH 45min)的pH值呈负相关。此外,LDHBPFKFB3的表达与死后48小时(DL 48h)测得的滴水损失和死后24小时(DL 24h)测得的滴水损失呈正相关。我们共同确定了一系列DEGs作为影响GP的潜在关键候选基因,并发现LDHBPFKFB3与GP和GP相关性状密切相关。我们的结果为深入研究猪的GP和GP相关性状的调控机制奠定了坚实的基础。

更新日期:2020-09-26
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