Pogonatherum crinitum is a promising lead (Pb) hyperaccumulator; however, the effects of Pb contamination on P. crinitum rhizosphere soil enzymatic activities and microbial composition remain largely unexplored. Thus, an indoor experiment was conducted by cultivating P. crinitum seedlings and exposing them to four Pb concentrations (0, 1,000, 2000 and 3000 mg/kg Pb). Protease, urease, acid phosphatase and invertase activities were determined using standard methods while soil bacterial composition was determined by 16 S rDNA sequencing. The results showed that rhizosphere soil acid phosphatase activity significantly increased with increasing Pb concentration, while urease activity was significantly greater in rhizosphere soil contaminated with 1000 and 2000 mg/kg than in the control. There was a clear shift in bacterial composition during phytoremediation by P. crinitum. Compared to the control, Bacteroidetes was more abundant in all Pb-contaminated soils, Actinobacteria was more abundant in 1000 mg/kg Pb-treated soil, and Firmicutes was more abundant in 3000 mg/kg Pb-treated soil. Positive correlations were observed between dominant bacterial phyla and soil enzyme activities. Metabolic pathways, such as ABC transporter, quinine reductase, and ATP-binding protein were significantly increased in rhizosphere soil bacteria with Pb contamination. In conclusion, Pb contamination differentially influenced the activities of rhizosphere soil enzymes, specifically increasing acid phosphatase and urease activities, and alters the dominance of soil bacteria through up-regulation of genes related to some metabolic pathways. The strong correlations between dominant bacterial phyla and enzymatic activities suggest synergetic effects on the growth of P. crinitum during Pb contamination.

Pogonatherum crinitum是一种有前途的铅（Pb）超富集剂。然而，Pb污染对P. crinitum根际土壤酶活性和微生物组成的影响仍未开发。因此，通过栽培景天假单胞菌进行了室内实验。幼苗并使其暴露于四种Pb浓度（0、1,000、2000和3000 mg / kg Pb）。蛋白酶，脲酶，酸性磷酸酶和转化酶的活性用标准方法测定，而土壤细菌组成则通过16 S rDNA测序测定。结果表明，随着铅浓度的增加，根际土壤酸性磷酸酶活性显着增加，而被1000和2000 mg / kg污染的根际土壤中的脲酶活性明显高于对照。在由P. crinitum进行的植物修复过程中细菌组成发生了明显变化。与对照相比，在所有受Pb污染的土壤中，拟杆菌含量较高，在1000 mg / kg铅处理过的土壤中放线菌更丰富，在3000 mg / kg铅处理过的土壤中菌丝更丰富。显性细菌菌群与土壤酶活性之间存在正相关。在具有铅污染的根际土壤细菌中，代谢途径（例如ABC转运蛋白，奎宁还原酶和ATP结合蛋白）显着增加。总之，铅污染对根际土壤酶的活性有不同的影响，特别是增加酸性磷酸酶和脲酶的活性，并通过上调与某些代谢途径有关的基因来改变土壤细菌的优势。铅污染期间的P. crinitum。