Contamination by organic and inorganic compounds remains one of the most complex problems in both brackish and marine environments, causing potential implications for the reproductive success and survival of several broadcast spawners. Ficopomatus enigmaticus is a tubeworm polychaete that has previously been used as a model organism for ecotoxicological analysis, due to its sensitivity and ecological relevance. In the present study, the effects of five trace elements (zinc, copper, cadmium, arsenic and lead), one surfactant (sodium dodecyl sulfate, SDS) and one polycyclic aromatic hydrocarbon (benzo(a)pyrene, B(a)P) on the sperm quality of F. enigmaticus were investigated. Sperm suspensions were exposed in vitro to different concentrations of each selected contaminant under four salinity conditions (10, 20, 30, 35). Possible adverse effects on sperm function were assessed by measuring oxidative stress, membrane integrity, viability and DNA damage. Sperm quality impairments induced by organic contaminants were more evident than those induced by inorganic compounds. SDS exerted the largest effect on sperm. In addition, F. enigmaticus sperm showed high tolerance to salinity variation, supporting the wide use of this species as a promising model organism for ecotoxicological assays. Easy and rapid methods on polychaete spermatozoids were shown to be effective as integrated sperm quality parameters or as an alternative analysis for early assessment of marine and brackish water pollution.

在咸淡和海洋环境中，有机和无机化合物的污染仍然是最复杂的问题之一，对数个繁殖产卵者的繁殖成功和生存造成潜在影响。Ficopomatus enigmaticus是一种块虫多毛cha，由于其敏感性和生态相关性，以前已被用作生态毒理分析的模型生物。在本研究中，五种微量元素（锌，铜，镉，砷和铅），一种表面活性剂（十二烷基硫酸钠，SDS）和一种多环芳烃（苯并（a）py，B（a）P）的影响对迷迭香的精子质量进行了研究。精子悬浮液在体外暴露在四种盐度条件下（10、20、30、35）达到每种选定污染物不同的浓度。通过测量氧化应激，膜完整性，生存力和DNA损伤来评估对精子功能可能产生的不利影响。由有机污染物引起的精子质量损伤比由无机化合物引起的精子质量损伤更为明显。SDS对精子的影响最大。此外，F。enigmaticus精子对盐分变化表现出高度的耐受性，支持该物种作为生态毒理学测定中的一种有希望的模式生物而得到广泛使用。事实证明，简单快速的多壳类精子方法可作为综合的精子质量参数或作为早期评估海洋和微咸水污染的替代分析方法有效。