In order to study the effects and mechanism of florfenicol (FFC) on the kidney function of broilers, 180 1-day-old broilers were randomly divided into 6 groups, 30 in each group. Except for the control group, different doses of FFC were added to drinking water in the other 5 groups (0.15 g/L, 0.3 g/L, 0.6 g/L, 1.2 g/L and 1.8 g/L). After continuous administration for 5 days, renal histopathological changes, serum renal function indicators, renal peroxidation products and antioxidant factors, and apoptotic factors were detected in broilers aged 21 and 42 days. The results showed that compared with the control group, the kidney tissue structure was disordered, the glomerulus was atrophic, the cystic cavity was enlarged, and the epithelial cells of renal tubules were seriously vacuolated in broilers of treatment groups. And with the growth of broilers, the kidney injury of broilers in the low-dose FFC group was relieved. FFC significantly increased the contents of uric acid (UA), blood urea nitrogen (BUN), creatinine (CRE) in serum and malondialdehyde (MDA) in kidney of broilers, but significantly reduced the levels of glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) in kidney. FFC significantly inhibited the mRNA relative transcriptional levels of nuclear factor-erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1) and nicotinamide adenine dinucleotide phosphate: quinone oxidoreductase-1 (NQO-1), and increased the mRNA and protein expression levels of p53, Caspase-3 and Caspase-6 in kidney tissue of broilers. It is concluded that FFC has certain nephrotoxicity to broilers, and its effect on kidney is dose-dependent and reversible. FFC causes intense lipid peroxidation in broiler kidney by inhibiting the expression of related factors in the downstream signal pathway of Nrf2. FFC can also up-regulate the expression of pro-apoptotic factors and accelerate the abnormal apoptosis of renal cells, thus seriously affecting the renal function of broilers.

为了研究氟苯尼考（FFC）对肉仔鸡肾脏功能的影响和作用机理，将180只1日龄肉仔鸡随机分为6组，每组30只。除对照组外，其他5组分别向饮用水中添加了不同剂量的FFC（0.15 g / L，0.3 g / L，0.6 g / L，1.2 g / L和1.8 g / L）。连续给药5天后，在21和42天的肉鸡中检测到肾脏组织病理学变化，血清肾功能指标，肾脏过氧化产物和抗氧化因子以及凋亡因子。结果表明，治疗组肉鸡与对照组相比，肾脏组织结构紊乱，肾小球萎缩，囊腔扩大，肾小管上皮细胞严重空泡。随着肉鸡数量的增长，低剂量FFC组肉鸡的肾脏损伤得到缓解。FFC显着增加了肉鸡肾脏中尿酸（UA），血尿素氮（BUN），肌酐（CRE）和丙二醛（MDA）的含量，但显着降低了谷胱甘肽（GSH），超氧化物歧化酶（SOD）的水平）和肾脏中的过氧化氢酶（CAT）。FFC显着抑制核因子-类胡萝卜素2相关因子2（Nrf2），血红素加氧酶-1（HO-1）和烟酰胺腺嘌呤二核苷酸磷酸：醌氧化还原酶-1（NQO-1）的mRNA相对转录水平，并增加肉仔鸡肾脏组织中p53，Caspase-3和Caspase-6的mRNA和蛋白表达水平。结论是，FFC对肉鸡具有一定的肾毒性，其对肾脏的作用具有剂量依赖性和可逆性。FFC通过抑制Nrf2下游信号通路中相关因子的表达，在肉鸡肾脏中引起强烈的脂质过氧化。FFC还可以上调促凋亡因子的表达并加速肾细胞​​的异常凋亡，从而严重影响肉鸡的肾功能。