We have developed a versatile label-free surface-enhanced Raman spectroscopic platform for detecting various biotargets via proximity hybridization-triggered DNA assembly based on the 736 cm-1 Raman peak of adenine breathing mode. We initially immobilized the first probe to AuNPs and modified the second with poly adenine. Presence of target DNA or protein molecules assembled a sandwich complex that brought the poly adenine close to the AuNPs surface, generating Raman signals, that were proportional to target molecule concentration. These approach exhibits high sensitivity, with a detection limit of 5.4 pM, 47 fM, and 0.51 pg/mL for target DNA, thrombin and CEA, respectively. Owing to a one step proximity dependent complex formation, this technique is simple and can be completed within 40 min, making it a promising candidate for point-of-care testing applications.

中文翻译：

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用于无标记表面增强拉曼光谱生物分析的邻近杂交触发 DNA 组装

我们开发了一种多功能的无标记表面增强拉曼光谱平台，用于通过基于腺嘌呤呼吸模式的 736 cm-1 拉曼峰的邻近杂交触发 DNA 组装检测各种生物目标。我们最初将第一个探针固定在 AuNPs 上，然后用聚腺嘌呤修饰第二个探针。目标 DNA 或蛋白质分子的存在组装了一个夹心复合物，使聚腺嘌呤靠近 AuNPs 表面，产生与目标分子浓度成正比的拉曼信号。这些方法具有高灵敏度，对目标 DNA、凝血酶和 CEA 的检测限分别为 5.4 pM、47 fM 和 0.51 pg/mL。由于一步接近依赖复杂的形成，这种技术很简单，可以在 40 分钟内完成，