A novel Gram-positive and endospore-forming bacterium assigned as strain SPB7^T which is also a new source of a cyclic diketopiperazine (3S,6S)-3,6-diisobutylpiperazine-2,5-dione is described. A polyphasic (biochemical, phenotypic and genotypic) approach was used to clarify the taxonomic affiliation of this strain. The partial and complete 16S rRNA gene sequences revealed that strain SPB7^T is a member of the Bacillus genus [showing high similarity (> 98.70%) with Bacillus spizizenii NRRL B-23049^T, Bacillus tequilensis KCTC 13622^T, Bacillus inaquosorum KCTC 13429^T and Bacillus cabrialesii TE3^T]. The maximum values for average nucleotide identity (ANI) and in silico DNA–DNA hybridization (GGDC, Formula 2) of strain SPB7^T was obtained for twenty-five strains of Bacillus spizizenii (ANI 95.01–95.48% and GGDC 62.70–60.00%). The whole-genome phylogenetic relationship showed that SPB7^T formed an individual and separated clade with the Bacillus spizizenii group. Principal cellular fatty acids identified in strain SPB7^T were anteiso C_15:0, anteiso C_17:0, iso C_15:0, iso C_17:0, C_16:0, C_10:0 3OH and iso C_{17:1 ϖ10c}. Polar lipid profile showed presence of diphosphotidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unknown phospholipids and five unknown lipids. Cells were rod shaped, catalase, oxidase-positive and motile. Growth occurred at 20–45 °C (optimal 35 °C), at pH 6.0–10.0 (optimal pH 8) and 0–10% (w/v) NaCl (optimal 2%). The phenotypic, biochemical, and genotypic traits of strain SPB7^T strongly supported its taxonomic affiliation as a novel species of the Bacillus genus, for which the name Bacillus rugosus sp. nov. is proposed. The type strain is SPB7^T (= NRRL B-65559^T, = CICC 24827^T, = MCC 4185^T).

描述了一种新的革兰氏阳性和内生孢子细菌，命名为SPB7 ^T菌株，它也是环二酮哌嗪（3S，6S）-3,6-二异丁基哌嗪-2,5-二酮的新来源。多相（生化，表型和基因型）方法用于阐明该菌株的分类学隶属关系。在部分和完全的16S rRNA基因序列显示，应变SPB7 ^Ť是成员芽孢杆菌属[示出具有高的相似性（> 98.70％）芽孢杆菌小号pizizenii NRRL B-23049 ^Ť，芽孢杆菌tequilensis KCTC 13622 ^Ť，芽孢杆菌inaquosorum KCTC 13429 ^Ť和芽孢杆菌TE3 ^T ]。对于平均核苷酸同一性（ANI）和在计算机芯片上的DNA-DNA杂交的最大值（GGDC，式2）应变SPB7的^Ť是为25株获得的芽孢杆菌小号pizizenii（ANI 95.01-95.48％和GGDC 62.70-60.00％ ）。的全基因组的亲缘关系显示SPB7 ^Ť形成为与单独的和分离的进化枝芽孢杆菌小号pizizenii基。在应变SPB7鉴定主要细胞脂肪酸^Ť分别为反异Ç _15：0，反异Ç _17：0，ISO C _15：0，ISO C _17：0，C_16：0，C _10：0 3OH和iso C _{17：1 ϖ10c}。极性脂质谱显示存在二磷脂酰甘油，磷脂酰乙醇胺，磷脂酰甘油，两个未知的磷脂和五个未知的脂质。细胞为棒状，过氧化氢酶，氧化酶阳性和运动。生长在20–45°C（最佳35°C），pH 6.0–10.0（最佳pH 8）和0–10％（w / v）NaCl（最佳2％）下发生。SPB7 ^T菌株的表型，生化和基因型性状强烈支持其分类学隶属关系，是一种新的芽孢杆菌属，其名称为Bacillus rugosus sp。十一月 被提议。类型菌株为SPB7 ^T（= NRRL B-65559 ^T，= CICC 24827^T，= MCC 4185 ^T）。