当前位置: X-MOL 学术Plant Mol. Biol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Class I TCP proteins TCP14 and TCP15 are required for elongation and gene expression responses to auxin.
Plant Molecular Biology ( IF 5.1 ) Pub Date : 2020-09-15 , DOI: 10.1007/s11103-020-01075-y
Lucia V Ferrero 1 , Victoria Gastaldi 1 , Federico D Ariel 1 , Ivana L Viola 1 , Daniel H Gonzalez 1
Affiliation  

Key Message

Two class I TCP transcription factors are required for an efficient elongation of hypocotyls in response to auxin and for the correct expression of a subset of auxin-inducible genes

Abstract

In this work, we analyzed the response to auxin of plants with altered function of the class I TEOSINTE BRANCHED 1, CYCLOIDEA, PCF (TCP) transcription factors TCP14 and TCP15. Several SMALL AUXIN UP RNA (SAUR) genes showed decreased expression in mutant plants defective in these TCPs after an increase in ambient temperature to 29 °C, a condition that causes an increase in endogenous auxin levels. Overexpression of SAUR63 caused a more pronounced elongation response in the mutant than in the wild-type at 29 °C, suggesting that the decreased expression of SAUR genes is partly responsible for the defective elongation at warm temperature. Notably, several SAUR genes and the auxin response gene IAA19 also showed reduced expression in the mutant after auxin treatment, while the expression of other SAUR genes and of IAA29 was not affected or was even higher. Expression of the auxin reporter DR5::GUS was also higher in a tcp15 mutant than in a wild-type background after auxin treatment. However, the elongation of hypocotyls in response to auxin was impaired in the mutant. Remarkably, a significant proportion of auxin inducible genes and of targets of the AUXIN RESPONSE FACTOR 6 are regulated by TCP15 and often contain putative TCP recognition motifs in their promoters. Furthermore, we demonstrated that several among them are recognized by TCP15 in vivo. Our results indicate that TCP14 and TCP15 are required for an efficient elongation response to auxin, most likely by regulating a subset of auxin inducible genes related to cell expansion.



中文翻译:

I 类 TCP 蛋白 TCP14 和 TCP15 是对生长素的延伸和基因表达反应所必需的。

关键信息

两种 I 类 TCP 转录因子是有效延长下胚轴以响应生长素和正确表达生长素诱导基因子集所必需的

抽象的

在这项工作中,我们分析了 I 类 TEOSINTE BRANCHED 1、CYCLOIDEA、PCF (TCP) 转录因子 TCP14 和 TCP15 功能改变的植物对生长素的反应。在环境温度升高到 29 °C 后,一些SMALL AUXIN UP RNA ( SAUR ) 基因在这些 TCP 缺陷的突变植物中的表达降低,这种情况会导致内源性生长素水平升高。SAUR63的过表达导致突变体在 29 °C 下比野生型更明显的延伸反应,这表明SAUR基因的表达降低是温暖温度下延伸缺陷的部分原因。值得注意的是,几个SAUR基因和生长素反应基因IAA19还显示生长素处理后突变体中的表达降低,而其他SAUR基因和IAA29的表达不受影响甚至更高。生长素报告基因DR5::GUStcp15中的表达也较高与生长素处理后的野生型背景相比,突变体。然而,响应于生长素的下胚轴伸长在突变体中受损。值得注意的是,很大比例的生长素诱导基因和 AUXIN RESPONSE FACTOR 6 的靶标受 TCP15 调节,并且通常在其启动子中包含推定的 TCP 识别基序。此外,我们证明了其中的几个在体内被 TCP15 识别。我们的结果表明,TCP14 和 TCP15 是对生长素的有效延伸反应所必需的,很可能是通过调节与细胞扩增相关的生长素诱导基因的一个子集。

更新日期:2020-09-16
down
wechat
bug